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安全性情報

14385C

SAFC

EX-CELL® CD HEK293 Viral Vector Medium

Chemically defined, animal component-free, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

医薬品製造

別名:

HEK293/HEK293T medium for viral vector production

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About This Item

UNSPSCコード:
12352207

品質水準

500

無菌性

sterile-filtered

製品種目

EX-CELL®

フォーム

liquid

テクニック

cell culture | mammalian: suitable (suspension)

構成

L-glutamine: no

輸送温度

wet ice

保管温度

2-8°C

関連するカテゴリー

詳細

EX-CELL CD HEK293 Viral Vector Medium is an animal origin-free, chemically defined cell culture medium for clinical and commercial manufacturing of viral vectors for gene and cell therapies. The medium is intended for processes implementing (polyethylenimine) PEI transfection reagents. EX-CELL CD HEK293 Viral Vector Medium is part of the VirusExpress® Lentiviral Production Platform and the medium of choice for VirusExpress 293T Lentiviral Production Cells (VP001). The medium is also suitable as a standalone solution for other HEK293 and HEK293T cell lines for AAV and lentiviral manufacturing. EX-CELL CD HEK293 Viral Vector Medium has been formulated to support high suspension cell densities with minimal clumping, high transient transfection efficiency when used with PEI, and high viral titers in shake flask and stirred tank bioreactor formats. Viral vector production using this medium is a scalable, suspension process that does not require a media exchange or feed post-transfection.

EX-CELL CD HEK293 Viral Vector Medium has been designed with long-term needs for manufacturing in mind, with a sustainable raw material supply chain, custom packaging options, and careful material and manufacturing process selection. The product is produced in an ISO9001-certified facility following the principles of cGMP according to IPEC-PQG.

アプリケーション

EX-CELL CD HEK293 Viral Vector Medium was developed to support VirusExpress Production Platforms. It performs well within the platform as well as with other HEK293 clones. The following white paper demonstrates the use of one of the prototype media formulations in developing a scalable, transient transfection-based suspension process for lentiviral vector production using the VirusExpress 293T Lentiviral Production cells:

法的情報

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
VirusExpress is a registered trademark of Merck KGaA, Darmstadt, Germany

保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

14385CPROC:
14385C-1000ML:
14385C-PH:
14385C-BULK:
14385C-10B:
14385C-1B:
14385C-20B:

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試験成績書（COA）

Lot/Batch Number
0000344163
0000324691
0000344163
0000256533
SLCP2393

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資料

Development and Optimization of a Lentivirus Manufacturing Platform

Learn how development of a suspension adapted HEK293T cell line with accompanying cell culture media can maximize lentivirus production for cell and gene therapies.

Scalability Challenges of Viral Vector Manufacturing

Scaling up viral vector production using adherent cell culture systems is challenging. Learn how suspension cell culture systems benefit large-scale bioprocessing.

Bioreactor Process Development and Scale-Up for Transient Transfection-Based Lentivirus Manufacturing in Suspension Cell Culture

A step-by-step overview of suspension-based, transient transfection bioreactor process development and scaleup of lentivirus production.

Bioprocessing And Scale-Up Of Suspension HEK293 Cells For AAV Manufacturing

To address scalability challenges of AAV manufacturing, we developed an HEK293 suspension cell line that can be used across many serotypes. Get the data in this article.

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関連コンテンツ

Viral Vector Upstream Platforms

A transfection-based solution to viral vector production using a suspension cell line, chemically defined medium, and a process with proven performance at scale.

ウイルスベクターアップストリームプロセス

浮遊培養用細胞株、CD (chemically defined) 培地、およびプロセススケールの性能が実証された、トランスフェクションによるウイルスベクター生産のソリューションです。

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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