A0349

Anti-l-Afadin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• MDL番号: MFCD03455360
• UNSPSCコード: 12352203
• NACRES: NA.41
• 結合体: unconjugated
• application: ARR; IF; IHC (f); WB
• クローン: polyclonal
• 化学種の反応性: canine, mouse, rat, human
• citations: 22
• テクニック: immunohistochemistry (frozen sections): 1:1,000 using mouse liver sections.; indirect immunofluorescence: 1:500 using MDCK cells and cultured human HepG2 cells.; microarray: suitable; western blot: 1:2,000 using extract of rat brain

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~200 kDa
• 化学種の反応性: canine, mouse, rat, human
• 包装: antibody small pack of 25 μL
• 強化検証: independent; Learn more about Antibody Enhanced Validation
• テクニック: immunohistochemistry (frozen sections): 1:1,000 using mouse liver sections.; indirect immunofluorescence: 1:500 using MDCK cells and cultured human HepG2 cells.; microarray: suitable; western blot: 1:2,000 using extract of rat brain
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... MLLT4(4301) ; mouse ... Mllt4(17356) ; rat ... Mllt4(26955)

詳細

詳細

Afadin is a multidomain protein that binds F-actin and E-catenin proteins at cell-cell adheren junctions. I-afadin is a ubiquitously expressed splice variant of afadin that contains PDZ and F-actin binding domains at the C-terminal end. I-afadin is homologous to the human AF-6 protein .

特異性

Anti-l-Afadin antibody is specific for I-afadin (approximately 200 kDa). It also binds I-afadin in rat, mouse, dog and human.

免疫原

Synthetic peptide sequence corresponding to amino acid residues 1814-1829 of rat afadin with N-terminal cysteine conjugated to maleimide-activated KLH.

アプリケーション

Anti-l-Afadin antibody produced in rabbit has been used in:

• western blotting
• immunofluorescence experiments
• immunocytochemistry
• immunohistochemistry

生物化学的／生理学的作用

AF-6/Afadin regulates the formation of tight and adherens junctions in the cell and its absence in mice leads to embryonic defects and lethality . Afadin is implicated in primary breast cancer and pancreatic cancer. It downregulates the process of migration and metastasis in cancer cells.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable