A2231

Anti-Axin1 (C-terminal region) ウサギ宿主抗体

~2.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: Anti-Axin, Anti-Axis Inhibitor 1, Anti-Axis inhibition protein 1
• MDL番号: MFCD02095870
• UNSPSCコード: 12352203
• NACRES: NA.41
• 結合体: unconjugated
• application: IHC; WB
• クローン: polyclonal
• 化学種の反応性: mouse, rat, human
• citations: 8
• テクニック: immunohistochemistry: suitable; western blot: 0.5-1 μg/mL using HEK-293 cells expressing mouse axin1

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~130 kDa
• 化学種の反応性: mouse, rat, human
• 濃度: ~2.5 mg/mL
• テクニック: immunohistochemistry: suitable; western blot: 0.5-1 μg/mL using HEK-293 cells expressing mouse axin1
• UniProtアクセッション番号: O15169
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... AXIN1(8312) ; mouse ... Axin1(12005) ; rat ... Axin1(79257)

詳細

詳細

Axin1 contains two conserved domains, an N-terminal regulator of G-protein signaling (RGS) domain, and a C-terminal DIX domain. The C-terminal region of axin1 is important for homodimerization, whereas the central region binds β-catenin and GSK-3b.

免疫原

synthetic peptide corresponding to amino acids 815-833 located near the C-terminal region of human axin1, conjugated to KLH. This sequence is identical in rat and mouse axin1 and highly conserved (83% identity) in human, rat, and mouse axin2.

アプリケーション

Immunohistochemistry was performed using rabbit anti-axin antibody to visualize Axin in the mouse developing cortex. Mouse brains had been previously harvested and fixed in 4% paraformaldhyde and cryoprotected in 20% sucrose. Axin was seen in the soma and axons of neurons.

Rabbit anti-axin1 (C-terminal region) antibody can be used at a concentration of 0.5-1μg/ml in western blot assays using HEK-293 cells expressing mouse axin1.

生物化学的／生理学的作用

Axin1 (axis inhibitor-1 or axin) functions as a negative regulator of Wnt signaling. Axin1 is known to associate with several members of the Wnt pathway such as β-catenin, glycogen synthase kinase-3b (GSK-3b), dishevelled (Dsh) and adenomatous polyposis coli (APC).

Axin1 (axis inhibitor-1, axin) enhances the phosphorylation of β-catenin by Glycogen synthase kinase 3 β (GSK-3b). Axin1 is involved in the degradation of β-catenin by acting as a scaffold to form a complex between β-catenin, adenomatous polyposis coli (APC) and GSK-3b thus, facilitating β-catenin phosphorylation by GSK-3b. Axin1 itself is phosphorylated and its activity regulated by GSK-3b. Axin1 phosphorylation is reduced by Wnt signaling, leading to decreased affinity of interaction of axin1 and β-catenin. This results in the release of β-catenin from the degradation complex and increased stability of β-catenin in the cell. Axin1 is destabilized by Wnt signaling and its levels in the cell are reduced. Axin isoform axin2 (conductin, axil) is 44% identical to Axin1 and appears to play a similar role to Axin1 in the Wnt signaling pathway.

物理的形状

Solution in 0.01 mM phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)