B3523

モノクロナール抗ヒトIgG3−ビオチン マウス宿主抗体

clone HP-6050, purified from hybridoma cell culture

• 別名: モノクロナール抗ヒトIgG3
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: biotin conjugate
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: secondary antibodies
• クローン: HP-6050, monoclonal
• フォーム: buffered aqueous solution
• 化学種の反応性: human
• テクニック: direct ELISA: 1:40,000
• アイソタイプ: IgG1
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified

詳細

詳細

Human IgG consists of four subclasses (1-4) that can be recognized by antigenic differences in their heavy chains. They constitute approximately 65, 30, 5 and 4% of the total IgG, respectively. Each subclass has different biological and physicochemical properties. The IgG subclass may be preferentially produced in response to different antigens and pathological conditions. For instance, anti-polysaccharide responses are mainly of the IgG2 subclass while protein antigens give rise to IgG1 and IgG3 antibodies.

Monoclonal Anti-Human IgG3 (mouse IgG1 isotype) is derived from the hybridoma (HP-60501) produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

特異性

本抗体は、ヒトIgG3に特異的であり、他のIgGサブクラスには反応しません。このクローンは、その特異性と反応性から、IUIS/WHOの研究によって幅広いアッセイプロトコールやテクニックへの応用が推奨されています。

免疫原

Purified myeloma human IgG3 coupled to polyaminostyrene (PAS) microbeads was used as the immunogen

アプリケーション

The product may be used for the identification of human IgG3 subclass by means of various immunoassays and ELISA. It has also been used in immunoblotting.

生物化学的／生理学的作用

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to monoclonal Anti-Human IgG3 antibody allows for the binding of various labels such as avidin or streptavidin.

Only IgG1 and IgG3 are capable of adherence to mononuclear phagocytes. IgG3 has O-glycosylation sites and mediates, phagocytosis, antibody-mediated cellular cytotoxicity, neutralization, and activates complement pathways. IgG3 subtypes have short in vivo half-life and mediates the effector functions than IgG1.

物理的形状

0.01 M PBS溶液(pH 7.4, 1% BSA, 15 mMアジ化ナトリウム含有)

保管および安定性

For continuous use, store at 2-8°C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

その他情報

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable