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Merck

F1773

Sigma-Aldrich

モノクロナール抗CD4

clone Q4120, purified from hybridoma cell culture

別名:

Monoclonal Anti-CD4

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About This Item

UNSPSCコード:
12352203
NACRES:
NA.44

由来生物

mouse

品質水準

結合体

FITC conjugate

抗体製品の状態

purified immunoglobulin

抗体製品タイプ

primary antibodies

クローン

Q4120, monoclonal

形状

buffered aqueous solution

分子量

antigen 59 kDa

化学種の反応性

human

テクニック

flow cytometry: 10 μL using 1 × 106 cells

アイソタイプ

IgG1

UniProtアクセッション番号

輸送温度

wet ice

保管温度

2-8°C

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... CD4(920)

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詳細

Anti-CD4-FITC antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cell line NS-1 and splenocytes from Balb/c mice immunized with cluster of differentiation 4 (CD4)-transfected mouse T cell hybridoma, 3DT, followed by CD4+ human T cell CEM cells (leukemic lymphoblasts).
CD4, a glycoprotein, is predominantly expressed on the cell membrane of mature, thymus-derived (T) lymphocyte and also to some extent in the monocyte/macrophage lineage. It has a transmembrane domain, cytoplasmic region and an extracellular region of four tandem domain similar like members of Ig superfamily.

特異性

CD4を認識します。クロ-ン番号Q4120モノクロ-ナル抗体が認識するエピト-プは、アミノ酸1-183に位置しており、ホルマリン固定およびパラフィン包埋の影響をうけます。5th Workshop: code no. CD04.11

免疫原

CD4をトランスフェクトしたマウスT細胞ハイブリド-マ3DT、続いてCD4+ヒトT細胞由来CEM細胞

アプリケーション

FITC Conjugated Monoclonal Anti-Human CD4 has been used for:
  • identification, quantification and monitoring of helper/inducer T cells in peripheral blood, biological fluids, lymphoid organs, and other tissues
  • analysis of T cell mediated cytotoxicity
  • characterization of subtypes of T cell leukemias and lymphomas
  • studies of T cells in health and disease
  • flow cytometry

生物化学的/生理学的作用

CD4+ T cells acts as a helper/inducer, in providing an activating signal to B cells. It induces T lymphocytes bearing the reciprocal CD8 marker to become cytotoxic/suppressor cells. It is also majorly associated with non-polymorphic region of MHC (major histocompatibility complex) antigen in interacting with targets bearing MHC class II molecules. Thus, it is called as an adhesion molecule. It also behaves as receptor for the GP 120 envelope glycoprotein of human immunodeficiency virus. The CD4 molecules is involved in the adhesion of T lymphocytes to target cells, thymic development, transmission of intracellular signals during T cell activation, and binding to polyclonal immunoglobulins.
Immunoregulatory T cell subset abnormalities in autoimmunity disorders, immunodeficiency diseases, graft versus host disease and following immunosuppressive therapy are often manifested as a change in CD4+/CD8+ ratio in peripheral blood T cells.

ターゲットの説明

CD4は、免疫グロブリン・ス-パ-ファミリ-に由来する単一鎖の膜貫通型糖タンパク質です。CD4は、ヘルパ-/インデュ-サ-T細胞サブセットをはじめ、大部分の胸腺髄質細胞、あるいはミクログリア細胞、樹状細胞やT細胞起源のある種の悪性腫瘍に発現しています。CD4は、MHCクラスII分子に結合し、p56lckプロテインチロシンキナ-ゼと結合します。

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

調製ノート

Prepared by conjugation to fluorescein isothiocyanate isomer I (FITC). This green dye is efficiently excited at 495 nm and emits at 525 nm.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

10 - Combustible liquids

WGK

nwg

引火点(°F)

Not applicable

引火点(℃)

Not applicable

個人用保護具 (PPE)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

F1773-50TST:
F1773-BULK:
F1773-100TST:
F1773-VAR:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Variation of blood T lymphocyte subgroups in patients with non-small cell lung cancer
Wang WJ, et al.
Asian Pacific Journal of Cancer Prevention, 14(8), 4671-4673 (2013)
Qi-Zhi Zhang et al.
Molecular medicine reports, 22(5), 3629-3634 (2020-10-02)
Phosphoinositide 3-kinase catalytic subunit δ isoform (P110δ) is mainly expressed in white blood cells. It is involved in T and B lymphocyte differentiation, maturation and the neutrophil chemotaxis process. Apolipoprotein E (ApoE) is an arginine‑rich alkaline protein, which is present
Belete A Desimmie et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 20(11), 2064-2075 (2012-07-26)
The interaction between the human immunodeficiency virus (HIV) integrase (IN) and its cellular cofactor lens epithelium-derived growth factor (LEDGF/p75) is crucial for HIV replication. While recently discovered LEDGINs inhibit HIV-1 replication by occupying the LEDGF/p75 pocket in IN, it remained
Angelo Gallina et al.
The Journal of biological chemistry, 277(52), 50579-50588 (2002-09-10)
We previously reported that monoclonal antibodies to protein-disulfide isomerase (PDI) and other membrane-impermeant PDI inhibitors prevented HIV-1 infection. PDI is present at the surface of HIV-1 target cells and reduces disulfide bonds in a model peptide attached to the cell
The production of armed effector T cells
Janeway, et al.
Immunobiology (2001)

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