L3410

抗レプチン ウサギ宿主抗体

affinity isolated antibody, buffered aqueous solution

• 別名: 抗OB抗体
• MDL番号: MFCD00803408
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 化学種の反応性: mouse, human
• テクニック: indirect ELISA: 1:1,000 using human or mouse recombinant leptin; microarray: suitable; western blot: 1:5,000 using human recombinant leptin
• UniProtアクセッション番号: P41159
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... LEP(3952) ; mouse ... Lep(16846)

詳細

詳細

The gene for leptin (LEP) is mapped to the human chromosome 7q32.1. The encoded protein is a non-glycosylated polypeptide, primarily produced and secreted by the mature adipocytes. Leptin is expressed in white adipose tissue, fetal tissues, skeletal muscle, chondrocytes, gastric chief cells, the placenta, ovary, and many immune cells. It is absent or expressed at extremely low levels in other adult tissues. Human leptin is 85% identical to mouse leptin and 84% identical to rat leptin, while mouse and rat exhibit 96% identity in their leptin molecules.

特異性

Anti-Leptin reacts specifically with leptin.

免疫原

ヒトおよびマウスレプチンのN末端アミノ酸配列22-40に相当する合成ペプチド。

アプリケーション

Anti-Leptin antibody produced in rabbit has been used in:

• western blotting
• immunohistochemistry
• immunocytochemistry
• enzyme linked immunosorbent assay (ELISA)

生物化学的／生理学的作用

Leptin is a circulating cytokine that induces pleiotropic effects on nutrient intake, thermogenesis, metabolism, reproduction and hematopoiesis. Leptin is hypothesized to be a “satiety factor” because the absence of this factor is associated with hyperphagia and obesity in ob/ob mice (homozygous for the obese spontaneous mutation). It appears that leptin is involved in appetite regulation since leptin injection into ob/ob mice reduces their food intake and ultimately their body weight. However, studies have demonstrated that its effects must be more complicated than simple appetite suppression.

Leptin is a hormone secreted by adipocytes and regulates energy homeostasis, inflammation and neuroendocrine processes, synaptic formation and memory condensation. The physiological effects of leptin are mediated by leptin receptors, ObR and LepR. Leptin is the key regulator of obesity and acts directly on neuronal networks to regulate feeding, metabolism and gastric mobility. The pathways that mediate the effect of leptin are Janus kinase (JAK)/signal transducer and activator of transcription (STAT), phosphoinositide 3-kinase (PI3K) and c-Jun N-terminal kinase (JNK).

物理的形状

PBS溶液 (1% BSA, 15mMアジ化ナトリウム含有)。

調製ノート

抗体はアガロ-ス固定ペプチドでアフィニティ-分離しています。

保管および安定性

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable