MAK493

Isocitrate Dehydrogenase (IDH) Assay Kit

sufficient for 100 colorimetric tests

• UNSPSCコード: 12352204
• NACRES: NA.84

特性

• 品質水準: 100
• 検出方法: colorimetric
• 関連疾患: hematological disorder; cancer
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

Isocitrate dehydrogenase (IDH) is an enzyme which catalyzes the interconversion of isocitrate and α-ketoglutarate. There are three IDH isoforms: IDH3 uses the cofactor NAD⁺ and catalyzes the third step in the citric acid cycle, while IDH1 and IDH2 use the cofactor NADP⁺ and catalyze the same reaction outside the citric acid cycle. This kit measures the activity of the IDH1 and IDH2 isoforms. Mutations in IDH1 and IDH2 have been linked with various brain tumors and acute myeloid leukemia.

特徴および利点

• Linear detection range 0.1 to 100 U/L for 30 minute reaction.
• Compatible with high-throughput handling systems.

適合性

The kit is suitable for IDH activity determination in biological samples (e.g. plasma, serum, tissue and culture media.)

原理

The Isocitrate Dehydrogenase (IDH) Assay Kit is a non-radioactive, colorimetric IDH assay based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT, which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.

安全性情報

• ピクトグラム: GHS02,GHS05,GHS07
• シグナルワード: Danger
• 危険有害性情報: H225,H290,H315,H319,H336
• 注意書き: P210 - P233 - P234 - P240 - P303 + P361 + P353 - P305 + P351 + P338
• 危険有害性の分類: Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
• ターゲットの組織: Respiratory system
• 保管分類コード: 3 - Flammable liquids
• WGK: WGK 2