R4654

Anti-Rab5 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: Anti-Ras-Related Protein Rab5A
• MDL番号: MFCD01322589
• UNSPSCコード: 12352203
• NACRES: NA.46

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~24 kDa
• 化学種の反応性: human, mouse, rat
• 濃度: ~1 mg/mL
• テクニック: indirect immunofluorescence: 5-10 μg/mL using rat NRK and mouse NIH-3T3 cells.; western blot: 2.5-5 μg/mL using whole extract of human A431 cells
• UniProtアクセッション番号: P20339
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... RAB5A(5868) ; mouse ... Rab5a(271457) ; rat ... Rab5a(64633)

詳細

詳細

Ras related protein consists of three isoforms such as Rab5a, Rab5b and Rab5c. Rab5a is encoded by the gene mapped to human chromosome 3p24.3. This gene codes for 23.66 kDa Rab5a protein.

免疫原

synthetic peptide corresponding to amino acid residues 176-188 of human Rab5 with C-terminal added cysteine, conjugated to KLH.

アプリケーション

Anti-Rab5 antibody produced in rabbit has been used in western blotting and immunocytochemistry.

生物化学的／生理学的作用

Rab proteins are members of small GTPases control the exocytosis and endocytosis and regulate vesicular transport. Rab5 regulates endocytic pathway in mammalian cells and an activator of autophagy. It mediates macroautophagy of cells expressing aggregates of mutant Huntingtin protein.

Rab5a is essential for epidermal growth factor (EGF)-mediated EGF receptor degradation. Overexpression of Rab5a is associated with the development of variety of cancers such as breast cancer, lung cancer, hepatocellular carcinoma (HCC) and ovarian cancer.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable