S8321

Monoclonal Anti-Sonic Hedgehog (Shh) マウス宿主抗体

clone SH154, purified from hybridoma cell culture

• 別名: Anti-Shh
• MDL番号: MFCD06798717
• UNSPSCコード: 12352203
• NACRES: NA.43
• 結合体: unconjugated
• application: ELISA (i); IHC; WB
• クローン: SH154, monoclonal
• 化学種の反応性: human, rat, mouse
• citations: 10
• テクニック: immunohistochemistry: 1:500 using formaldehyde-fixed human tissue sections; indirect ELISA: 2 μg/mL; western blot: 2 μg/mL using recombinant mouse SHH peptide

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified immunoglobulin
• 抗体製品タイプ: primary antibodies
• クローン: SH154, monoclonal
• フォーム: buffered aqueous solution
• 化学種の反応性: human, rat, mouse
• 濃度: ~2 mg/mL
• テクニック: immunohistochemistry: 1:500 using formaldehyde-fixed human tissue sections; indirect ELISA: 2 μg/mL; western blot: 2 μg/mL using recombinant mouse SHH peptide
• アイソタイプ: IgG1
• UniProtアクセッション番号: Q15465
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... SHH(6469) ; mouse ... Shh(20423) ; rat ... Shh(29499)

詳細

詳細

The Hedgehog (Hh) family of secreted proteins consists of sonic hedgehog (Shh), desert hedgehog (Dhh) and Indian hedgehog (Ihh). The Hh signaling regulates cell migration and tissue construction during embryogenesis and reconstruction in adults. Shh protein is secreted by Purkinje cells in the cerebellum with both autocrine and paracrine functions. The Shh signaling is mediated by the interaction of Shh with transmembrane proteins, Patched and Smoothened. The Shh pathway cross-talks Notch and Wnt pathways and acts via downstream targets such as cyclins, γ catenin, myc and other transcription factors. The Hh family members have multiple roles as morphogens, cell survival factors, setting anterior-posterior axis and digit formation during development of limb and axon guidance factors during the development of brain and nervous system. Hedgehog signaling has been implicated in childhood tumors of pancreas, liver, thyroid, gastrointestinal tract, neuroblastomas and medulloblastomas
Anti-Sonic Hedgehog antibody recognizes human, rat and mouse Shh.

特異性

Mouse monoclonal clone SH154 anti-Sonic Hedgehog antibody recognizes human, rat, and mouse Shh.

アプリケーション

Anti-Sonic Hedgehog antibody may be used for immunoblotting at a working concentration of ~2 μg/ml. A working antibody concentration of ~2 μg/ml is recommended for ELISA. For immunohistochemistry a dilution of 1:500 may be used.

Mouse monoclonal clone SH154 anti-Sonic Hedgehog antibody may be used in ELISA, immunoblotting, and immunohistochemistry. It is used as a probe to determine the presence and roles of Sonic Hedgehog in processes such as patterning during embryogenesis.

生物化学的／生理学的作用

Sonic Hedgehog (Shh) is an important cell signaling molecule expressed during embryonic development. Shh is involved in patterning of the developing embryonic systems such as the nervous system, somites, and limbs. The N-terminal peptide of Shh is released by autoproteolysis and functions through interactions with a multicomponent receptor complex containing the transmembrane proteins Patched and Smoothened. Shh protein is expressed in key embryonic tissues such as the Hensen′s node, zone of polarizing activity in the posterior limb bud, notochord, and floor plate of the neural tube. Downstream targets of Shh include the transcription factors Gli3, responsible for Greigs polycephalosyndactyly in humans, and Hoxd13, responsible for polysyndactyly.

物理的形状

Solution in 0.01 M phosphte buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)