SAB4200005

モノクローナル抗UVRAG抗体 マウス宿主抗体

clone UVRAG-11, purified from hybridoma cell culture

• 別名: 抗DHTX抗体, 抗UV照射耐性関連遺伝子タンパク質抗体, 抗p63抗体
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: mouse
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: UVRAG-11, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen ~90 kDa
• 化学種の反応性: mouse, human
• 濃度: ~1.0 mg/mL
• テクニック: immunoprecipitation (IP): suitable; western blot: 2-4 μg/mL using whole extract of human G361 cells
• アイソタイプ: IgG1
• UniProtアクセッション番号: Q9P2Y5
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... UVRAG(7405) ; mouse ... Uvrag(78610)

詳細

詳細

Monoclonal Anti-UVRAG (mouse IgG1 isotype) is derived from the hybridoma UVRAG-11 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human UVRAG.

Ultraviolet irradiation resistance-associated gene (UVRAG) is a crucial autophagic tumor suppressor, encoded by the gene mapped to human chromosome 11q13.5. The encoded protein is characterized with four functional domains, such as proline-rich domain, a lipid-binding C2 domain, a Beclin1-binding coiled-coil domain (CCD) and a C-terminal domain involved in centrosome integrity and DNA damage repair.

アプリケーション

Monoclonal Anti-UVRAG antibody produced in mouse has been used in immunoprecipitation.

Monoclonal Anti-UVRAG antibody produced in mouse has been used in western blotting and immunohistochemistry.

生物化学的／生理学的作用

UVRAG is positive regulator of the Beclin 1- class III phosphatidylinositol 3-kinase (PI(3)KC3) complex. The tumor suppressor Beclin 1 forms a complex with PI(3)KC3, promoting autophagosome formation. UVRAG interacts with Beclin 1 through their coiled-coil domains and induces autophagy resultin in suppression of proliferation and tumorigenicity of human colon cancer cells. UVRAG is a tumor suppressor candidate.

Ultraviolet irradiation resistance-associated gene (UVRAG) is implicated in the regulation of intracellular membrane trafficking, including autophagy and chromosomal stability. The encoded protein regulates apoptosis by suppressing the BCL2-associated X protein (Bax) activity. Mutation in the gene has been observed in various types of human cancers, including microsatellite unstable colon carcinomas.

ターゲットの説明

Anti-UVRAG, UV radiation resistance associated gene, complements the ultraviolet sensitivity of xeroderma pigmentosum group C cells and encodes a protein with a C2 domain. The protein activates the Beclin1-PI(3)KC3 complex, promoting autophagy and suppres

物理的形状

0.01 M PBS溶液(pH 7.4, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable