SAB4200017

モノクローナル抗MUC1抗体 マウス宿主抗体

clone 1D1, purified from hybridoma cell culture

• 別名: 抗CD227抗体, 抗EMA抗体, 抗H23AG抗体, 抗KL-6抗体, 抗MAM6抗体, 抗PEMT抗体, 抗PEM抗体, 抗PUM抗体, 抗ムチン1抗体
• UNSPSCコード: 12352203
• NACRES: NA.41
• 結合体: unconjugated
• application: ELISA (i); ICC; IP; RIA; WB
• クローン: 1D1, monoclonal
• 化学種の反応性: human
• citations: 4
• テクニック: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; radioimmunoassay: suitable; western blot: 0.5-1.0 μg/mL using T47D cell lysates

特性

• 由来生物: mouse
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified from hybridoma cell culture
• 抗体製品タイプ: primary antibodies
• クローン: 1D1, monoclonal
• フォーム: buffered aqueous solution
• 分子量: antigen 160-500 kDa
• 化学種の反応性: human
• 濃度: ~1.0 mg/mL
• テクニック: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; radioimmunoassay: suitable; western blot: 0.5-1.0 μg/mL using T47D cell lysates
• アイソタイプ: IgG1
• UniProtアクセッション番号: P15941
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... MUC1(4582)

詳細

詳細

Monoclonal Anti-MUC1 (mouse IgG1 isotype) is derived from the hybridoma 1D1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with semi-purified MUC1 from pleural effusion of a breast cancer patient. The Mucin 1 (MUC1) gene encodes a membrane bound glycosylated phosphoprotein, belonging to the family of mucins which are heavily glycosylated proteins. Muc1 is a type I transmembrane glycoprotein with a unique extracellular domain containing 20 amino acid variable number tandem repeat (VNTR) domain. In many epithelia, this protein is attached to the apical surface by a transmembrane domain. The degree of glycosylation varies with cell type. The MUC1 gene is mapped to human chromosome 1q22.

免疫原

乳癌患者の胸水由来MUC1の準精製物。

アプリケーション

Monoclonal Anti-MUC1 antibody produced in mouse has been used in:

• immunoblotting
• enzyme linked immunosorbent assay (ELISA)
• radioimmunoassay
• immunoprecipitation
• immunocytochemistry

生物化学的／生理学的作用

Mucin 1 (Muc1) exhibits protective action by binding to pathogens and is also associated with cell signaling capacity. Upregulation of this gene, abnormal intracellular localization and varied glycosylation of Muc1 has been associated with carcinomas. Muc1 might serve as a candidate for single chain antibody mediated targeted therapy in MUC1 expressing cancers. They are preferentially expressed by a variety of adenocarcinomas, including breast, prostate, ovarian and pancreatic carcinomas as well as malignant plasma cells of multiple myelomas.

物理的形状

0.01 M PBS溶液(pH 7.4, 15mMアジ化ナトリウム含有)。

法的情報

Purchase of this product conveys a non-exclusive license to use the product for research purposes only. Purchase of this product does not convey a right to (i) incorporate or use the product in the manufacture of any commercial product, (ii) sell or otherwise transfer the product to any third party, or (iii) use the product to provide any commercial service. THE PRODUCT IS NOT FOR IN VIVO HUMANS USE.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable