P4832

Poly-ʟ-Lysine Hydrobromide

synthetic, mol wt 150,000-300,000, solution, 0.01%, suitable for cell culture

• Synonym(s): PLL Solution
• CAS Number: 25988-63-0
• MDL number: MFCD00165424
• UNSPSC Code: 12352202
• eCl@ss: 32160406
• NACRES: NA.75

Properties

• Product Name: Poly-L-lysine solution, mol wt 150,000-300,000, 0.01%, sterile-filtered, BioReagent, suitable for cell culture
• biological source: synthetic
• sterility: sterile-filtered
• product line: BioReagent
• form: solution
• mol wt: 150,000-300,000
• packaging: pkg of 50 mL
• concentration: 0.01%
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested

Description

General description

L-lysine is a homopolypeptide having two enantiomeric forms, L and D lysine. Through its three reactive groups (the carboxylic acid and the α and ε amines), L-lysine has the capacity to generate various branched polymeric structures, including dendrimers, branched polylysine, and hyperbranched polylysine (PLL). [1] Poly-L-lysine is a polymer made up of positively charged amino acid residues, with each lysine residue typically containing one HBr.

Application

Poly-L-lysine has been used:

• In coating 96-well plates for cell attachment in testing respiratory burst activity.[2]
• In coating glass cover slips for immunofluorescent labeling of intracellular structures.[3]
• For pretreating 6-well plate in myosatellite cell isolation protocol.[4]
• In coating coverslips for cell adhesion in E14 neuron culture to study possible mechanism of action of circRNA_01477 in neurons.[5]

Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.

Biochem/physiol Actions

Poly‐l‐lysine (PLL) acts as polycationic polymers that have been extensively used to carry drugs, genes etc. across the cell membrane. [6] Poly-L-lysine (PLL) has the capability to engage in the formation of polyelectrolyte complexes (PECs) by interacting with anionic entities such as polyanions, nucleic acid chains and some drugs.[6]

Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.

Components

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Preparation Note

This product is supplied as a 0.01% sterile filtered solution in water, using a molecular weight of 150 - 300 kDa. Coating slides with this solution should be followed with a 5 minute incubation and drying the slides at room temperature. Coated slides will be stable for one year if protected from dust.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable