159220
Diethyl pyrocarbonate
96% (NT)
동의어(들):
DEP, DEPC, Diethyl dicarbonate, Diethyl oxydiformate, Ethoxyformic acid anhydride
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모든 사진(1)
About This Item
Linear Formula:
O(COOC2H5)2
CAS Number:
Molecular Weight:
162.14
Beilstein:
637031
EC Number:
MDL number:
UNSPSC 코드:
12352100
PubChem Substance ID:
NACRES:
NA.22
추천 제품
Quality Level
분석
96% (NT)
양식
liquid
refractive index
n20/D 1.398 (lit.)
bp
93-94 °C/18 mmHg (lit.)
density
1.101 g/mL at 25 °C (lit.)
작용기
carbonate
배송 상태
wet ice
저장 온도
2-8°C
SMILES string
CCOC(=O)OC(=O)OCC
InChI
1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3
InChI key
FFYPMLJYZAEMQB-UHFFFAOYSA-N
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일반 설명
Diethyl pyrocarbonate reacts selectively with the single-stranded loops of cruciform structures. The irreversible inactivation of ribonuclease with diethylpyrocarbonate has been studied.
애플리케이션
Diethyl pyrocarbonate was used as:
- nuclease inhibitor for the extraction of undegraded nucleic acids from etiolated and green plant tissues
- an antimicrobial additive
- ribonuclease inhibitor
- histidine residue modifier
- reagent for conversion of imines to carbamates
Modification reagent for His and Tyr residues in proteins. Robust probe for structural disruptions in dsDNA, reacting with fully or partially unstacked bases.
생화학적/생리학적 작용
Inactivates RNase in solution at about 0.1% (v/v), thus protecting RNA against degradation.
신호어
Warning
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Acute Tox. 4 Oral
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
156.2 °F - closed cup
Flash Point (°C)
69 °C - closed cup
개인 보호 장비
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Tetrahedron, 48, 3445-3445 (1992)
P D Pelton et al.
The Journal of biological chemistry, 267(9), 5916-5920 (1992-03-25)
The pH dependence of myo-inositol monophosphatase may indicate a role for histidine residues in the catalytic mechanism (Ganzhorn, A. J., and Chanal, M.-C. (1990) Biochemistry 29, 6065-6071). This possibility was investigated by chemical modification. At pH 6.0 and 25 degrees
Heather L Walsh et al.
PloS one, 15(8), e0236104-e0236104 (2020-08-11)
There is an increasing emphasis on effects-based monitoring to document responses associated with exposure to complex mixtures of chemicals, climate change, pathogens, parasites and other environmental stressors in fish populations. For decades aquatic monitoring programs have included the collection of
Isolation of plant RNA.
R Hodge
Methods in molecular biology (Clifton, N.J.), 28, 37-40 (1994-01-01)
A mechanism of the irreversible inactivation of bovine pancreatic ribonuclease by diethylpyrocarbonate. A general reaction of diethylpyrocarbonate . A general reaction of diethylpyrocarbonate with proteins.
B Wolf et al.
European journal of biochemistry, 13(3), 519-525 (1970-04-01)
문서
Solid-state lithium fast-ion conductors are crucial for safer, high-energy-density all-solid-state batteries, addressing conventional battery limitations.
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