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Merck
모든 사진(1)

Key Documents

906557

Sigma-Aldrich

DLAM-LVproS-13CH3 Methyl Labeling Kit

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모든 사진(1)

About This Item

UNSPSC 코드:
12352200
NACRES:
NA.12

기술

bio NMR: suitable

Quality Level

200

배송 상태

dry ice

저장 온도

−70°C

관련 카테고리

일반 설명

DLAM-LVproS-13CH3 has 13CH3 isotopomer precursors and contains protocol instructions for creation of isotopically-labeled proteins.

애플리케이션

For detection of long-range nOes or for the NMR study of large proteins
Methyl groups of leucine and valine precursors is labeled with 13CH3 isotopomer using DLAM-LVproS-13CH3 kit. Amino-acid selective labeling combined with modern NMR techniques is frequently employed as a useful tool for resolving resonance assignments. This kit has been tested with protein isotopic labeling in E. coli. DLAM-LVproS-13CH3 is used for optimal resonance dispersion of supramolecular protein structure in solution NMR spectroscopy.

포장

This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

픽토그램

Corrosion
GHS05

신호어

Danger

유해 및 위험 성명서

H314

Hazard Classifications

Skin Corr. 1B

Storage Class Code

8A - Combustible corrosive hazardous materials

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

Kit I Tong et al.
Journal of biomolecular NMR, 42(1), 59-67 (2008-09-03)
A simple and user-friendly method of labeling protein selectively with amino acids in vivo is introduced. This technique does not require the use of transaminase-deficient or auxotrophic strains. By manipulating the product feedback inhibitory loops of the E. coli amino
Stereospecific isotopic labeling of methyl groups for NMR spectroscopic studies of high-molecular-weight proteins.
Pierre Gans et al.
Angewandte Chemie (International ed. in English), 49(11), 1958-1962 (2010-02-17)
Rime Kerfah et al.
Current opinion in structural biology, 32, 113-122 (2015-04-17)
Nuclear magnetic resonance (NMR) spectroscopy is a uniquely powerful tool for studying the structure, dynamics and interactions of biomolecules at atomic resolution. In the past 15 years, the development of new isotopic labeling strategies has opened the possibility of exploiting
Silke Wiesner et al.
Current opinion in structural biology, 35, 60-67 (2015-09-26)
Intermolecular interactions are indispensible for biological function. Here we discuss how novel NMR techniques can provide unique insights into the assembly, dynamics and regulation of biomolecular complexes. We focus on applications that exploit the methyl TROSY effect and show that

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