04-1571-I

Anti-RNA polymerase II subunit B1 (phospho CTD Ser-2), clone 3E10 (rat monoclonal).

culture supernatant, clone 3E10, from rat

• 동의어(들): DNA-directed RNA polymerase II subunit RPB1, RNA polymerase II subunit B1, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase III largest subunit, RNA-directed RNA polymerase II subunit RPB1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rat
• Quality Level: 100
• 항체 형태: culture supernatant
• 항체 생산 유형: primary antibodies
• 클론: 3E10, monoclonal
• 종 반응성: mouse
• 종 반응성(상동성에 의해 예측): human (immunogen homology)
• 기술: ChIP: suitable; ELISA: suitable; western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_000928
• UniProt 수납 번호: P24928
• 배송 상태: wet ice
• 타겟 번역 후 변형: phosphorylation (pSer2)
• 유전자 정보: human ... POLR2B(5431)

설명

일반 설명

RPB1 (RNA polymerase II subunit B1) is the catalytic component of RNA polymerase II which synthesizes mRNA and non-coding RNAs. During transcription, elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of RPB1, which acts as an assembly platform for factors regulating transcription initiation, elongation, termination and mRNA processing. Phosphorylation occurs mainly at residues ′Ser-2′ and ′Ser-5′ of the tandem 7 residue repeats in the C-terminal domain (CTD), which activates Pol II. This phosphorylation also can occur at ′Ser-7′ of the heptapepdtide repeat. This antibody recognizes the ′Ser-2′ CTD residue of RPB1.

특이성

Demonstrated to react with human RBP1 in Ni et al., 2011. Transcription 2:5, 237-242

This antibody recognizes RNA Polymerase II subunit B1 phosphorylated at Ser2 of the C-terminal domain (CTD).

면역원

Epitope: Phosphorylated Ser2 at the C-terminal domain (CTD)

Ovalbumin-conjugated linear peptide corresponding to human RNA Polymerase II subunit B1 phosphorylated at Ser2 of the C-terminal domain (CTD).

애플리케이션

Anti-RNA polymerase II subunit B1 (phospho CTD Ser-2), clone 3E1 (rat monoclonal) is a highly specific rat monoclonal antibody & that targets RNA Polymerase & has been tested in western blotting.

Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to immunoprecipitate RNA polymerase II subunit B1 (phospho-CTD Ser-5) in ChIP (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.).
Demonstrated to react with human RBP1 in Ni et al., 2011. Transcription 2:5, 237-242

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

품질

Evaluated by Western Blotting in untreated and lambda phosphatase treated NIH/3T3 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected RNA Polymerase II subunit B1 (phospho CTD Ser-2) in untreated NIH/3T3 cell lysate.

표적 설명

~220 kDa observed

결합

Replaces: 04-1571

물리적 형태

Unpurified

Unpurified rat monoclonal IgG1κ tissue culture supenatant with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

분석 메모

Control
Untreated and lambda phosphatase treated NIH/3T3 cell lysate

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1