04-797

Anti-phospho-MAP Kinase 1/2 (Erk1/2)(Thr185/Tyr187) Antibody, clone AW39

clone Aw39, Upstate^®, from rabbit

• 동의어(들): Mitogen-activated protein kinase, Extracellular signal-regulated kinase, protein tyrosine kinase ERK2
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: culture supernatant
• 항체 생산 유형: primary antibodies
• 클론: Aw39, monoclonal
• 종 반응성: human, rat
• 제조업체/상표: Upstate^®
• 기술: multiplexing: suitable; western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NM_002745; NM_002746
• UniProt 수납 번호: P28482
• 배송 상태: dry ice
• 타겟 번역 후 변형: phosphorylation (pThr185/pTyr187)
• 유전자 정보: human ... MAPK1(5594) ; rat ... Mapk1(116590)

설명

일반 설명

Erk (Extracellular signal-Related Kinase) is a family of two, highly homologous proteins denoted as Erk1 (p44, MAPK3) and Erk2 (p42, MAPK1) that both function in the same pathway. The two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase. The Erk pathway is considered the classical, canonical MAPK (Mitogen-Activated Protein Kinase) signaling pathway. It is an evolutionarily conserved pathway that controls and is a critical regulator the growth and survival through the promotion of cell proliferation and the prevention of apoptosis. Erk is involved in the control of many fundamental cellular processes including cell proliferation, survival, differentiation, apoptosis, motility and metabolism. Erk is activated by growth factor stimulation of receptor tyrosine kinases (RTKs) and/or integrin stimulation. This activates the Ras-Raf-MEK,-Erk pathway that results in MEK, a dual kinase (a Ser/Thr and a Tyr kinase), phosphorylation and activation of ERK1/2 (p44/044) on the TxY motif (Thr202/Tyr204 and Thr185/Tyr187 for Erk1 and Erk2, respectively).

특이성

Predicted to cross-react with many species based on sequence homology.

Recognizes phosphorylated MAP Kinase 1 and 2 (Erk 1&2).

면역원

KLH-conjugated, synthetic peptide corresponding to amino acids surrounding the pTEpY motif of human phospho-MAP Kinase 1/2 (Erk1/2), in which the Thr and Tyr residues are phosphorylated. The immunizing sequence is highly conserved among species.

애플리케이션

Research Category
Signaling

Research Sub Category
MAP Kinases

Western Blot Analysis:
A 1:2,000-1:8,000 dilution of this lot detected phospho-MAP Kinase 1/2 (Erk1/2) in RIPA lysates from NGF stimulated PC-12 cells (Figure A).
Beadlyte Phospho-Specificity Assay:
1:500-1:4,000 dilutions of a previous lot detected active but not unactive MAP Kinase/Erk2 conjugated to Luminex microspheres. (Figure B).

품질

Routinely evaluated by immunoblot on RIPA lysates from NGF stimulated PC-12 cells

표적 설명

MAPK1 (~44 kDa) and MAPK2 (~42 kDa).

결합

Replaces: 05-797

물리적 형태

Cultured supernantant in 0.05% sodium azide

Format: Purified

저장 및 안정성

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

분석 메모

Control
RIPA cell lysate from NGF stimulated PC-12 cells

기타 정보

Concentration: Variable

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable