05-516

Anti-Ras Antibody, clone RAS10

clone RAS10, Upstate^®, from mouse

• 동의어(들): GTP- and GDP-binding peptide B, GTPase Hras, Ha-Ras1 proto-oncoprotein, Ras family small GTP binding protein H-Ras, Transforming protein p2, c-has/bas p21 protein, c-ras-Ki-2 activated oncogene, p19 H-RasIDX protein, transformation gene: oncogene HAMSV,
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: RAS10, monoclonal
• 종 반응성: rat, human, mouse
• 제조업체/상표: Upstate^®
• 기술: ELISA: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG2aκ
• NCBI 수납 번호: NM_005343
• UniProt 수납 번호: P01116
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... HRAS(3265) , KRAS(3845) , NRAS(4893)

설명

일반 설명

The tumor oncoproteins HRas, KRas, and NRas are rated as Ras protoncogenes. Point mutations within Ras genes are frequently detected in human malignancies and in experimentally induced tumors in animals.

특이성

Other species unknown.

면역원

Purified recombinant Ras. Clone RAS10.

애플리케이션

Detect Ras using this Anti-Ras Antibody, clone RAS10 validated for use in ELISA, FC, IC, IH, IP & WB.

Immunoprecipitation:
1-4 μg of this antibody has been reported to immunoprecipitate Ras.

Immunocytochemistry:
2.5 μg/mL of this antibody has been reported to show positive immunostaining for Ras in SW480 and Y1 cells.

Immunohistochemistry:
This antibody has been reported to detect Ras in frozen and paraffin embedded breast carcinoma sections.

품질

Routinely evaluated by western blot on RIPA lysate from human A431 carcinoma cells, human HFF, Jurkat, Raji, mouse 3T3, CTLL, rat L-6 or PC-12 cell lysates.

Western Blot Analysis:
0.05 - 0.5 μg/mL of this lot detected Ras in RIPA lysate from human A431 carcinoma cells. A previous lot detected Ras in human HFF, Jurkat, Raji, mouse 3T3, CTLL, rat L-6 and PC- 12 cell lysates.

표적 설명

21 kDa

결합

Replaces: CBL751

물리적 형태

Format: Purified

Purified mouse monoclonal IgG2ak in buffer containing PBS, pH 7.4, with 0.05% sodium azide and 30% glycerol. Liquid at -20°C.

분석 메모

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2