06-229

Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody

Upstate^®, from rabbit

• 동의어(들): CD340 antigen, MLN 19, NEU proto-oncogene, Tyrosine kinase-type cell surface receptor HER2, c-erb B2/neu protein, neuroblastoma/glioblastoma derived oncogene homolog, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma der
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human, rat
• 포장: antibody small pack of 25 μL
• 제조업체/상표: Upstate^®
• 기술: immunocytochemistry: suitable; western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NM_004448
• UniProt 수납 번호: P04626
• 배송 상태: ambient
• 타겟 번역 후 변형: phosphorylation (pTyr1248)
• 유전자 정보: human ... ERBB2(2064)

설명

일반 설명

The ErbB-2 proto-oncogene, also called Neu, EGFR-2 or HER-2, is a member of the transmembrane receptor tyrosine kinase family, which also includes EGF receptor and EGFR-3 (HER-3 or ErbB-3). Although, ErbB-2 does not have any known high-affinity ligands, its kinase activity can be activated without ligand by either overexpression or heteroassociation with other members of the ErbB family. Amplification of the ErbB-2 gene and overexpression of its product has been detected in almost 40% of primary human breast tumors, which correlates with poor prognosis in node positive breast cancer. ErbB-2 overexpression is also observed in ovarian, gastric, salivary and non-small cell lung carcinomas. Because of this, ErbB-2 is one of the major drug targets for breast cancer and other cancer treatments.

특이성

Predicted to cross react with rat based on conservation of immunogenic sequence.

Recognizes and is specific for the activated form of erbB-2/HER-2 oncoprotein, MW ~138 kDa.

면역원

Epitope: Tyr1248

Synthetic phosphopeptide, residues 1243-1255, corresponding to a major auto-phosphorylation (Tyr1248) site of human erbB-2/HER-2 (C-AENPEpYLGLDVPV).

애플리케이션

Immunohistochemistry:
IHC was performed by an independent laboratory.

Immunocytochemistry:
2 µg/mL of a previous lot showed positive immunostaining for phospho-erbB2/HER-2 in EGF treated A431 cells.

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

Kinases & Phosphatases

This Anti-phospho-erbB-2/HER-2 (Tyr1248) Antibody is validated for use in IC, WB for the detection of phospho-erbB-2/HER-2 (Tyr1248).

품질

Evaluated by Western Blot on A431+/-EGF lysates.

Western Blotting Analysis:
1:1000 dilution of this antibody detected phospho-erbB2/Her2 on 10 µg of A431+EGF lysates.

표적 설명

185 kDa

물리적 형태

Double immunoaffinity purified rabbit polyclonal IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH7.4

ImmunoAffinity Purified

저장 및 안정성

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

분석 메모

Control
EGF treated A431 cells

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable