추천 제품
![Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2 clone 7F9.2, from mouse](/deepweb/assets/sigmaaldrich/product/images/307/874/7354f72d-80ee-40a5-b7fa-0590fe6784cc/640/7354f72d-80ee-40a5-b7fa-0590fe6784cc.jpg)
생물학적 소스
rabbit
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
rabbit, human, rat
포장
antibody small pack of 25 μg
제조업체/상표
Upstate®
기술
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... CDKN1B(1027)
일반 설명
p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4. p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm.
특이성
Recognizes Kip1.
면역원
An 18 amino acid synthetic peptide ([C]GSVEQTPKKPGLRRRQT) linked to KLH was used to immunize rabbits
애플리케이션
Anti-Kip1 (p27) Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Kip1 (p27) & has been validated in IHC, IP & WB.
품질
routinely evaluated by immunoblot on HeLa nuclear extract or in rabbit brain nuclear extract
표적 설명
27 kDa
결합
Replaces: 04-240
물리적 형태
Format: Purified
분석 메모
Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Masahiro Morita et al.
Molecular and cellular biology, 27(13), 4980-4990 (2007-04-25)
The stability of mRNA influences the abundance of cellular transcripts and proteins. Deadenylases play critical roles in mRNA turnover and thus are important for the regulation of various biological events. Here, we report the identification and characterization of CCR4b/CNOT6L, which
High-throughput screening reveals alsterpaullone, 2-cyanoethyl as a potent p27Kip1 transcriptional inhibitor.
Walters, BJ; Lin, W; Diao, S; Brimble, M; Iconaru, LI; Dearman, J; Goktug, A; Chen, T; Zuo, J
Testing null
Protein phosphatase PP6 N terminal domain restricts G1 to S phase progression in human cancer cells.
Bjarki Stefansson et al.
Cell cycle (Georgetown, Tex.), 6(11), 1386-1392 (2007-06-15)
Yeast SIT4 is an essential gene encoding a protein Ser/Thr phosphatase conserved throughout eukaryotic evolution, known as PPV in Drosophila and PP6 in vertebrates. Sit4 promotes transcription of G1 cyclins and a sit4(ts) strain exhibits a G1 arrest at the
Forkhead transcription factor FoxO1 transduces insulin-like growth factor's signal to p27Kip1 in primary skeletal muscle satellite cells.
Shuichi Machida, Espen E Spangenburg, Frank W Booth
Journal of Cellular Physiology null
Cell cycle. Stifled by inhibitions.
Peters, G
Nature, 371, 204-205 (1994)
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