07-049
Anti-Nedd4
Upstate®, from rabbit
동의어(들):
E3 ubiquitin-protein ligase NEDD4, EC: 2.3.2.26, Cell proliferation-inducing gene 53 protein, HECT-type E3 ubiquitin transferase NEDD4, Neural precursor cell expressed developmentally down-regulated protein 4
로그인조직 및 계약 가격 보기
모든 사진(2)
About This Item
UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41
추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
rat
종 반응성(상동성에 의해 예측)
human (based on 100% sequence homology)
제조업체/상표
Upstate®
기술
immunohistochemistry: suitable (paraffin)
western blot: suitable
동형
IgG
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... NDFIP1(80762)
일반 설명
E3 ubiquitin-protein ligase NEDD4 (UniProt: P46934; also known as EC: 2.3.2.26, Cell proliferation-inducing gene 53 protein, HECT-type E3 ubiquitin transferase Nedd4, Neural precursor cell expressed developmentally down-regulated protein 4, NEDD-4) is encoded by the Nedd4 (also known as KIAA0093, Nedd4-1) gene (Gene ID: 4734) in human. NEDD4 is a peripheral membrane protein that is recruited to the plasma membrane by GRB10. Once complexed with GRB10 and IGF1R, it follows IGF1R internalization and remains associated with early endosomes. Nedd4 can accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. It is shown to specifically ubiquitinate Lysine 63 in target proteins. It also monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. NEDD4 is reported to polyubiquitinate the epithelial sodium channel and any mutations that disrupt association between Nedd4 and sodium channel can lead to Liddle s syndrome, a hyperaldosteronism-like hypertension disease. NEDD4 can interact with viral proteins that contain a late-budding motif P-P-P-Y and this interaction is believed to be essential for viral particle budding of many retroviruses, like HTLV-1 Gag, and MLV Gag. It contains four WW domains (WW 1-4; aa 610-643; 767-800; 840-873; and 892-925) and one HECT domain (aa 984-1318). The WW domains mediate its interaction with PPxY motif-containing proteins.
특이성
This rabbit polyclonal antibody specifically detects E3 ubiquitin-protein ligase Nedd4.
면역원
GST/His-tagged recombinant fragment corresponding to 68 amino acids from the N-terminal half of human Nedd4.
애플리케이션
Anti-Nedd4 Antibody, Cat. No. 07-049, is a rabbit polyclonal antibody that detects Nedd4 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative detected NEDD44 in rat cerebral cortex, rat cerebellum, and human colon cancer tissue sections.
품질
Evaluated by Western Blotting in rat kidney tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Nedd4 in rat kidney tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Nedd4 in rat kidney tissue lysate.
표적 설명
~110 kDa observed; 102.40 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
물리적 형태
Format: Purified
Purified rabbit polyclonal antibody in buffer containing 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 0.05% sodium azide.
분석 메모
Control
Kidney cytoplasmic extracts.
Kidney cytoplasmic extracts.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Nedd4 augments the adaptive immune response by promoting ubiquitin-mediated degradation of Cbl-b in activated T cells.
Yang, B; Gay, DL; MacLeod, MK; Cao, X; Hala, T; Sweezer, EM; Kappler, J; Marrack, P; Oliver, PM
Nature Immunology null
NEDD4-1 is a proto-oncogenic ubiquitin ligase for PTEN.
Wang, X; Trotman, LC; Koppie, T; Alimonti, A; Chen, Z; Gao, Z; Wang, J; Erdjument-Bromage et al.
Cell null
Upregulation of the E3 ligase NEDD4-1 by oxidative stress degrades IGF-1 receptor protein in neurodegeneration.
Kwak, YD; Wang, B; Li, JJ; Wang, R; Deng, Q; Diao, S; Chen, Y; Xu, R; Masliah, E; Xu et al.
The Journal of Neuroscience null
Kinase-independent feedback of the TAK1/TAB1 complex on BCL10 turnover and NF-?B activation.
Moreno-Garcia, ME; Sommer, K; Rincon-Arano, H; Brault, M; Ninomiya-Tsuji, J; Matesic et al.
Molecular and cellular biology null
Nicola Amodio et al.
The American journal of pathology, 177(5), 2622-2634 (2010-10-05)
Loss of the PTEN tumor suppressor gene occurs frequently in non-small-cell lung carcinoma (NSCLC), although neither genetic alterations nor epigenetic silencing are significant predictors of PTEN protein levels. Since recent reports implicated neural precursor cell expressed, developmentally down-regulated 4-1 (NEDD4-1)
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