07-1817

Anti-phospho-SRPK2 (Ser494) Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Serine/threonine-protein kinase SRPK2, SFRS protein kinase 2, Serine/arginine-rich protein-specific kinase 2, SR-protein-specific kinase 2
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human, mouse
• 종 반응성(상동성에 의해 예측): Xenopus (based on 100% sequence homology), rat (based on 100% sequence homology)
• 기술: inhibition assay: suitable (peptide); western blot: suitable
• NCBI 수납 번호: NP_872633
• UniProt 수납 번호: P78362
• 배송 상태: wet ice
• 타겟 번역 후 변형: phosphorylation (pSer494)
• 유전자 정보: human ... SRPK2(6733)

설명

일반 설명

Along with Serine/threonine protein kinase 2 (SRPK2), SRPK1 phosphorylates serine residues found in RS-domain-containing proteins, such as SFRS1 and SFRS2. These kinases also phosphorylate the same serine residues on the HBV core protein both in vitro and in vivo. The cytoplasmic proteins are critical for nuclear import of SR proteins in a phosphorylation-dependent manner. Additionally, they mediate the trafficking of splicing factors and play a role in spliceosome assembly and mitogenesis.

특이성

This antibody is specific for the protein kinase domain of SRPK2 phosphorylated at Ser494, independent of observed phosphorylation at Ser497.

면역원

Epitope: Protein kinase domain

KLH-conjugated linear peptide corresponding to the protein kinase domain of SRPK2 phosphorylated at Ser494.

애플리케이션

Anti-phospho-SRPK2 (Ser494) Antibody is an antibody against phospho-SRPK2 (Ser494) for use in WB, PIA.

Peptide Inhibition Analysis: 1 µg/mL from a representative lot detected SRPK2 in 10 µg of insulin treated HepG2 cell lysate.

Research Category
Signaling

Research Sub Category
Cell Cycle, DNA Replication & Repair

품질

Evaluated by Western Blot in untreated and insulin treated HepG2 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected SRPK2 in 10 µg HepG2 cell lysate.

표적 설명

~120 kDa observed. Two isoforms at ~77 kDa and ~79 kDa may be observed in some cell lysates.

물리적 형태

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Untreated and insulin treated HepG2 cell lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable