추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
serum
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성(상동성에 의해 예측)
human (based on 100% sequence homology)
기술
ChIP: suitable (ChIP-seq)
dot blot: suitable
immunocytochemistry: suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
acetylation (Lys5)
유전자 정보
human ... HIST2H4B(554313)
일반 설명
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs).
특이성
This antibody is expected to demonstrate broad species cross-reactivity.
This antibody recognizes the N-terminus of Histone H4 acetylated at Lys5.
면역원
Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-terminus of Histone H4 acetylated at Lys5.
애플리케이션
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected acetyl-Histone H4 in HeLa and A431 cells.
Dot Blot Specificity Analysis: A representative lot detected acetyl-histone H4 (Lys5) peptides, but not unacetylated-histone H4 (Lys4) peptides or other peptides corresponding to modified histones.
Chromatin Immunoprecipitation Analysis: 5 µL from a representative lot immunoprecipitated acetyl-Histone H4 in HeLa S3 chromatin.
Dot Blot Specificity Analysis: A representative lot detected acetyl-histone H4 (Lys5) peptides, but not unacetylated-histone H4 (Lys4) peptides or other peptides corresponding to modified histones.
Chromatin Immunoprecipitation Analysis: 5 µL from a representative lot immunoprecipitated acetyl-Histone H4 in HeLa S3 chromatin.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-acetyl-Histone H4 (Lys5) Antibody (Rabbit Polyclonal Antibody) validated in WB, DB, ICC, ChIP-seq, ChIP to detect acetyl-Histone H4 (Lys5) also known as H4K5Ac, Histone H4 (acetyl K5).
품질
Evaluated by Western Blot in HeLa acid extract.
Western Blot Analysis: A 1:2,000 dilution of this antibody detected acetyl-Histone H4 in 10 µg of HeLa acid extract.
Western Blot Analysis: A 1:2,000 dilution of this antibody detected acetyl-Histone H4 in 10 µg of HeLa acid extract.
표적 설명
~13 kDa observed
물리적 형태
Immunodepleted rabbit serum with 0.05% soduim azide and 30% glycerol.
Purified by immunodepletion
저장 및 안정성
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
분석 메모
Control
HeLa acid extract
HeLa acid extract
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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Nature communications, 6, 8856-8856 (2015-11-20)
Epigenetics plays critical roles in controlling stem cell self-renewal and differentiation. Histone H1 is one of the most critical chromatin regulators, but its role in adult stem cell regulation remains unclear. Here we report that H1 is intrinsically required in
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