추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
human, vertebrates
제조업체/상표
Upstate®
기술
ChIP: suitable (ChIP-seq)
dot blot: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
inhibition assay: suitable (peptide)
western blot: suitable
동형
IgG
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
monomethylation (Lys4)
유전자 정보
human ... H3C1(8350)
일반 설명
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
특이성
Broad species to cross-reactivity is expected.
Recognizes monomethyl-histone H3 (Lys4), Mr 17kDa.
면역원
Epitope: Lys4
KLH-conjugated, synthetic peptide containing the sequence ART[meK]…, in which meK corresponds to monomethyl-lysine at residue 4 of human histone H3.
애플리케이션
Anti-monomethyl-Histone H3 (Lys4) Antibody is a rabbit polyclonal antibody for detection of monomethyl-Histone H3 (Lys4) also known as H3K4me1, Histone H3 (mono methyl K4) & has been validated in ICC, IF, PIA, WB, ChIP-seq, DB.
Immunocytochemistry:
Reported by an independent laboratory.
Peptide Inhibition:
Specificity was confirmed by the ability of 1 μM of the immunizing peptide to completely abolish detection of histone H3 in immunoblot analysis of HeLa acid extracts. (see figure, lane 3). No competition was observed with peptides containing dimethyl-lysine 4, trimethyl-lysine 4, mono-methyllysine 4 or monomethyl-lysine 9 or 27.
Reported by an independent laboratory.
Peptide Inhibition:
Specificity was confirmed by the ability of 1 μM of the immunizing peptide to completely abolish detection of histone H3 in immunoblot analysis of HeLa acid extracts. (see figure, lane 3). No competition was observed with peptides containing dimethyl-lysine 4, trimethyl-lysine 4, mono-methyllysine 4 or monomethyl-lysine 9 or 27.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
품질
Routinely evaluated by Western Blot in acid extracted proteins from HeLa cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected methylated histone H3 in acid extracted proteins from HeLa cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected methylated histone H3 in acid extracted proteins from HeLa cells.
표적 설명
17 kDa
물리적 형태
Format: Purified
Protein A purified
Purified rabbit IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05 Sodium Azide, pH 7.4.
저장 및 안정성
Stable for 1 year at 2 to 8°C from date of receipt.
분석 메모
Control
HeLa cell extract
HeLa cell extract
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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