07-449

Anti-trimethyl-Histone H3 (Lys27) Antibody

Upstate^®, from rabbit

• 동의어(들): H3 histone family, member T, histone 3, H3, histone cluster 3, H3, H3k27me3
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: human, mouse
• 제조업체/상표: Upstate^®
• 기술: immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; multiplexing: suitable; western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NM_003493
• UniProt 수납 번호: Q16695
• 배송 상태: dry ice
• 타겟 번역 후 변형: trimethylation (Lys27)
• 유전자 정보: human ... HIST1H3F(8968)

설명

일반 설명

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

특이성

Broad species cross-reactivity expected.

Trimethylated histone H3 (Lys27). Broad species cross-reactivity expected.

면역원

KLH-conjugated, synthetic 2X-branched peptide containing the sequence …AR(me3K)SAP… in which me3K corresponds to trimethyl-lysine at residue 27 of human histone H3.

애플리케이션

Anti-trimethyl-Histone H3 (Lys27), also known as Anti-H3K27me3, is a highly published Rabbit Polyclonal Antibody. This protein A purified antibody is dot blot tested for trimethylated lysine 27 specificity and validated in WB, ICC, IP.

Immunocytochemistry:

Immunoprecipitation:

Beadlyte Histone-Peptide Specificity Assay:

품질

Routinely evaluated by western blot in acid extracted proteins from HeLa cells.

Western Blot Analysis:
0.4 µg/mL of anti-trimethyl histone H3 Lys27 was used on recombinant Histone H3 and HeLa cell acid extracts.

표적 설명

17 kDa

물리적 형태

Format: Purified

Purified rabbit IgG in buffer containing 0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl with 0.05% sodium azide before the addition of 30% glycerol.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1