추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
mouse, human, rat
제조업체/상표
Upstate®
기술
ChIP: suitable (ChIP-seq)
dot blot: suitable
flow cytometry: suitable
western blot: suitable
동형
IgG
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
acetylation (Lys9)
유전자 정보
human ... HIST1H3F(8968)
일반 설명
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
특이성
Histone H3 acetylated at position 9.
although this peptide sequence is identical in a wide range of animal and plant species.
면역원
Epitope: Surrounding H3K9ac
KLH-conjugated synthetic peptide (ARTKQTARK(Ac)STG-C) corresponding to amino acids 1-12 of Histone H3 with acetylated Lys9.
애플리케이션
Anti-acetyl-Histone H3 (Lys9) Antibody is a Rabbit Polyclonal Antibody for detection of Histone H3 acetylated on lysine 9. This purified Ab, also known as Anti-H3K9Ac, is specificity verified by dot blot (DB), published in peer reviewed journals, and validated in WB, ChIP, ChIP-seq, FC.
Chromatin Immunoprecipitation/ChIP-seq Analysis: A representative lot immunoprecipitated acetyl-Histone H3 (Lys9) in 5 µg of HeLa cell lysate.
Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected acetyl-Histone H3 (Lys9) in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
Flow Cytometry Analysis: 0.25 µg of this antibody from a representative lot detected acetyl-Histone H3 (Lys9) in 1X10E6 HEK293 cells.
Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected acetyl-Histone H3 (Lys9) in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
Flow Cytometry Analysis: 0.25 µg of this antibody from a representative lot detected acetyl-Histone H3 (Lys9) in 1X10E6 HEK293 cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
품질
Evaluated by Western Blotting in sodium butyrate treated HeLa cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected acetyl-Histone H3 (Lys9) in 10 µg of sodium butyrate treated HeLa cell lysate.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected acetyl-Histone H3 (Lys9) in 10 µg of sodium butyrate treated HeLa cell lysate.
표적 설명
~17 kDa observed. Uncharacterized band(s) may appear in some lysates.
결합
Replaces: 04-1003
물리적 형태
Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide with 30% glycerol.
저장 및 안정성
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Distinct patterns of histone methylation and acetylation in human interphase nuclei.
M Skalnikova, E Bartova, V Ulman, P Matula, D Svoboda, A Harnicarova, M Kozubek, S Kozubek
Physiological Research null
Myc and Miz-1 have coordinate genomic functions including targeting Hox genes in human embryonic stem cells.
Varlakhanova, N; Cotterman, R; Bradnam, K; Korf, I; Knoepfler, PS
Epigenetics & Chromatin null
N-Myc regulates expression of pluripotency genes in neuroblastoma including lif, klf2, klf4, and lin28b.
Cotterman, R; Knoepfler, PS
Testing null
M J Kruhlak et al.
The Journal of biological chemistry, 276(41), 38307-38319 (2001-08-02)
Histone acetylation, a reversible modification of the core histones, is widely accepted to be involved in remodeling chromatin organization for genetic reprogramming. Histone acetylation is a dynamic process that is regulated by two classes of enzymes, the histone acetyltransferases (HATs)
Positive histone marks are associated with active transcription from a methylated ICSBP/IRF8 gene.
Marina Tshuikina, Kenneth Nilsson, Fredrik Oberg
Gene null
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