07-626

Anti-phospho-AMPKα (Thr172) Antibody

Upstate^®, from rabbit

• 동의어(들): 5′-AMP-activated protein kinase, catalytic alpha-1 chain, AMP -activate kinase alpha 1 subunit, AMP-activated protein kinase, catalytic, alpha-1, AMPK alpha 1, AMPK alpha-1 chain, protein kinase, AMP-activated, alpha 1 catalytic subunit
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human
• 종 반응성(상동성에 의해 예측): bovine, rat, chicken, mouse, horse
• 제조업체/상표: Upstate^®
• 기술: western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NP_996790
• UniProt 수납 번호: Q13131
• 배송 상태: wet ice
• 타겟 번역 후 변형: phosphorylation (pThr172)
• 유전자 정보: human ... PRKAA1(5562) ; mouse ... Prkaa1(105787)

설명

일반 설명

AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and a key target for treating Type 2 diabetes and obesity. AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits (62 kDa) and non-catalytic beta and gamma subunits. The Alpha subunit has at least two isoforms (alpha 1 and alpha 2) that differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity.

특이성

This antibody recognizes AMPKα.

면역원

KLH-conjugated, synthetic peptide corresponding to amino acids surrounding phosphorylated Thr172 of human AMP-activated protein kinase alpha 2 (AMPKα2). The immunogenic site is conserved in AMPKα1, isoform 1 (Thr174), AMPKα1, isoform 2 (Thr189), and AMPKα2 (Thr172). Accession number NM_006251. The immunizing sequence is identical in rat.

애플리케이션

Anti-phospho-AMPKα (Thr172) Antibody is an antibody against phospho-AMPKα (Thr172) for use in WB.

Research Category
Signaling

Metabolism

Research Sub Category
Insulin/Energy Signaling

Western Blot (SNAP ID) Analysis:

품질

Evaluated by Western Blot in serum starved and serum starved, Lambda dephosphorylated HEK293 cell lysate.

Western Blot Analysis: A 1:500 dilution of this antibody detected AMPKα in serum starved and serum starved, Lambda dephosphorylated HEK293 cell lysate.

표적 설명

62 kDa

물리적 형태

Affinity purified

Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide.

저장 및 안정성

Maintain refrigerated at 2-8°C for 1 year from date of receipt.

분석 메모

Control
HEK293 cell lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable