09-310

Anti-phospho-EGFR (Tyr1069) Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Receptor tyrosine-protein kinase ErbB-1, avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, epidermal growth factor receptor, epidermal growth factor receptor (avian
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• 클론: polyclonal
• application: ELISA; ICC; WB
• 종 반응성: human
• 기술: ELISA: suitable; immunocytochemistry: suitable; western blot: suitable
• citations: 4

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human
• 종 반응성(상동성에 의해 예측): primate (based on 100% sequence homology), rat (based on 100% sequence homology), mouse (based on 100% sequence homology), pig (based on 100% sequence homology), horse (based on 100% sequence homology)
• 기술: ELISA: suitable; immunocytochemistry: suitable; western blot: suitable
• NCBI 수납 번호: NM_005228.3
• UniProt 수납 번호: P00533
• 배송 상태: wet ice
• 타겟 번역 후 변형: phosphorylation (pTyr1069)
• 유전자 정보: horse ... Egfr(100067755) ; human ... EGFR(1956) ; mouse ... Egfr(13649) ; pig ... Egfr(397070) ; rat ... Egf(25313)

설명

일반 설명

EGFR (Epidermal growth factor receptor, ErbB-1) is a receptor tyrosine kinase (RTK) that is one of four members of the EGFR/ErbB family of receptor tyrosine kinases. EGFR plays a key role in the regulation of essential normal cellular processes and in the pathophysiology of hyperproliferative diseases such as cancer. Activation of the EGFR signaling pathway has been linked with increased cell proliferation, angiogenesis, metastasis and decreased apoptosis. Upon ligand binding, EGFR undergoes a transition from an inactive monomeric form to an active dimer. In addition to forming homodimers, EGFR may pair with another member of the ErbB receptor family, such as ErbB2/Her2/neu, to create an activated heterodimer. EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity that leads to trans- and auto-phosphorylation of tyrosine residues within the cytoplasmic domain of the receptor tyrosine kinase of five tyrosine (Y) residues on sites Y992, Y1045, Y1068, Y1148 and Y1173 located on the C-terminal domain of EGFR. This autophosphorylation elicits downstream activation of several signal transduction cascades, principally the MAPK, Akt and JNK pathways through Ras, PI3 Kinase, and PLC pathways that ultimately leads to the activation of the various pathways downstream. Tyr1069 is phosphorylated during activation.

특이성

Reacts strongly and specifically with Tyr1069 phosphorylated EGFR

면역원

Epitope: Tyr1069 (cytoplasmic domain)

Tyrosine-phosphorylated epidermal growth factor receptor.

애플리케이션

Immunofluroescent Immunocytochemistry:

Research Category
Apoptosis & Cancer

Signaling

Research Sub Category
Kinases & Phosphatases

This Anti-phospho-EGFR (Tyr1069) Antibody is validated for use in ELISA, IC, WB for the detection of phospho-EGFR (Tyr1069).

품질

Routinely evaluated by Western Blot on untreated and EGF treated A431 lysates.

Western Blot Analysis:
1:1,000 dilution of this antibody detected Tyr1069 phosphorylated EGFR on 10 g of EGF treated A431 lysates.

표적 설명

~170 kDa

결합

Replaces: 04-339

물리적 형태

Antigen Affinity Purified

Antigen affinity purified rabbit polyclonal in 0.1M Tris-Glycine (pH 7.4) 150mM NaCl, with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.

분석 메모

Control
Untreated and EGF treated A431 lysates

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable