16-222

Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, FITC Conjugate

clone 3H10, Upstate^®, from mouse

• 동의어(들): H3 histone, family 3A, H3S10P, Histone H3 (phospho S10), H3 histone, family 3A, H3 histone, family 3B, H3 histone, family 3B (H3.3B)
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 결합: FITC conjugate
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 3H10, monoclonal
• 종 반응성: human
• 제조업체/상표: Upstate^®
• 기술: immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• 동형: IgG
• NCBI 수납 번호: NP_003484
• UniProt 수납 번호: Q16695
• 배송 상태: wet ice
• 타겟 번역 후 변형: phosphorylation (pSer10)
• 유전자 정보: human ... HIST1H3F(8968)

설명

일반 설명

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

특이성

Broad species cross-reactivity is expected.

Recognizes histone H3 phosphorylated at Ser10, MW 17 kDa.

면역원

A proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3. Clone 3H10.

Epitope: Ser10

애플리케이션

Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, FITC Conjugate is a Mouse Monoclonal for detection of Histone H3 phosphorylated at serine 10. This mAb, also known as H3S10p,is labeled with Fluorescein isothiocyanate (FITC), published in peer reviewed journals & validated in WB, ICC & IF.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Western Blot Analysis: 0.5-5 µg/mL of this lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells (Catalog # 17-306) treated with colcemid.

품질

Immunocytochemistry: Mitotic HeLa cells showed positive chromosome staining with 4μg/mL of this antibody.

표적 설명

17 kDa

물리적 형태

Protein G Purified

Purified mouse monoclonal IgG in buffer containing PBS with 0.05% sodium azide, pH7.1.

저장 및 안정성

Stable for 1 year at from date of receipt.

분석 메모

Control
Mitotic HeLa cells (IF).

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2