17-622

ChIPAb+ Trimethyl-Histone H3 (Lys27) - ChIP Validated Antibody and Primer Set

from rabbit, purified by using Protein A

• 동의어(들): Chip Antibody and primer set, H3K27me3 ChIP, H3K27me3, Histone H3 (tri methyl K27), Histone H3K27me3, Histone H3K27me3 ChIP
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.32

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 클론: polyclonal
• 정제법: using Protein A
• 종 반응성: human, mouse
• 제조업체/상표: ChIPAb+; Upstate^®
• 기술: ChIP: suitable; western blot: suitable
• NCBI 수납 번호: NP_003484
• UniProt 수납 번호: Q16695
• 배송 상태: dry ice
• 유전자 정보: human ... H3F3B(3021)

설명

일반 설명

All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot,
every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context.
Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ Trimethyl-Histone H3 (Lys27) set includes the Anti-Trimethyl-Histone H3 (Lys27) antibody, a negative control antibody (purified rabbit IgG), and qPCR primers which amplify a 139 bp region of human alpha-Satellite. The Trimethyl-Histone H3 (Lys27) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Trimethyl-Histone H3 (Lys27)-associated chromatin.

The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 27 of histone H3 can be mono-, di- or trimethylated (Histone H3 monomethyl Lys27, Histone H3 dimethyl Lys27 or Histone H3 trimethyl Lys27) by different histone methyltransferases such as EZH2 or NSD3. Methylation of this residue is mainly associated with transcriptional repression.

특이성

Broad species cross-reactivity expected.

trimethyl-Histone H3 (Lys27)

면역원

Epitope: Trimethyl Lys27

KLH-conjugated, synthetic 2X-branched peptide containing the sequence ...AR(me3K)SAP... in which me3K corresponds to trimethyl-lysine at residue 27 of human Histone H3.

애플리케이션

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from 2x10⁶ HeLa cells were subjected to chromatin immunoprecipitation using 4 μg purified anti-trimethyl-Histone H3 (Lys27) antibody or normal rabbit IgG and the Magna ChIP A kit (Cat. #17-610). Successful enrichment of trimethyl-Histone H3 (Lys27) associated DNA fragments was verified by qPCR using ChIP Primers GAPDH flanking the human GAPDH promoter and primers targeting the promoter of human MyoD.
Please refer to the EZ-Magna ChIP A (Cat. #17-408) or EZChIP (Cat. #17-371) kit protocols for experimental details.

Western Blot Analysis:
Recombinant Histone H3 (Lane 2) and HeLa cell acid extracts (Lane 1) were resolved by electrophoresis, transferred to nitrocellulose and probed with a 1:5000 dilution of anti- trimethyl histone H3 Lys27 (0.4 μg/mL).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Trimethyl-Histone H3 (Lys27) ChIP validated antibody & primer set including the ChIP-grade antibody & the specific control PCR primers used for chromatin immunoprecipitation of H3K27Me3.

포장

25 assays per kit, ~4μL per chromatin immunoprecipitation.

성분

Anti-trimethyl Histone H3(Lys27) polyclonal, 1 vial

Polyclonal control rabbit serum, 1 vial

Human alpha-Satellite primer set, 1 vial

품질

Chromatin Immunoprecipitation:
Sonicated Chromatin prepared from 3x10⁶ NIH3T3 L1 cells were subjected to chromatin immunoprecipitation using 4 μg purified antibody or normal rabbit IgG and the Magna ChIP^® A kit (Cat. #17-610). Successful enrichment of trimethyl-Histone H3
(Lys27) associated DNA fragments was verified by qPCR using ChIP Primers human alpha-Satellite (Please see figures).
Please refer to the EZ-Magna ChIP A (Cat. #17-408) or EZ-ChIP (Cat. #17-371) kit protocols for experimental details.

표적 설명

17kDa

물리적 형태

Anti-trimethyl-Histone H3 (Lys27) (rabbit polyclonal IgG). One vial containing 100 μg protein A purified IgG in 100 μL buffer containing 0.1 M Tris-Glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide. Store at -20°C.

Normal Rabbit IgG. One vial containing 125 μg purified Rabbit IgG in 125 μL storage buffer containing 0.1% sodium azide. Store at -20°C.

ChIP Primers human alpha-Satellite. One vial containing 75 μL of 5 μM of each control primer specific for human alpha-Satellite. Store at -20°C.
FOR: CTG CAC TAC CTG AAG AGG AC
REV: GAT GGT TCA ACA CTC TTA CA

저장 및 안정성

1 year at -20°C from date of shipment

분석 메모

Control
1 vial containing 75μl of 5μM of each control primer specific for human alpha-Satellite. Store at -20°C.

FOR: CTG CAC TAC CTG AAG AGG AC

REV: GAT GGT TCA ACA CTC TTA CA

법적 정보

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable