추천 제품
Quality Level
형태
liquid
제조업체/상표
Novagen®
저장 조건
OK to freeze
기술
transfection: suitable
배송 상태
wet ice
저장 온도
2-8°C
일반 설명
Stuart Rulten. Research Fellow. University of Sussex
""Since we have been working with GeneJuice, we have saved a lot of time and money!""
Dr. Andrea Kress, Institute of Clinical and Molecular Virology, Erlangen, Germany
""I find that Genejuice works for a multitude of cell types with minimal optimization""
Caitriona Marie Lyons. PhD Student. University College Cork, Ireland
Transfection is the process by which nucleic acids are introduced into mammalian cells. GeneJuice Transfection Reagent is a proprietary formulation optimized for maximal transfection efficiency, ease of use, and minimal cytotoxicity for mammalian cells. Whereas many available transfection reagents are based on cationic lipid formulation, GeneJuice Transfection Reagent is composed of a nontoxic cellular protein and a small amount of a novel polyamine. GeneJuice Transfection Reagent enables highly efficient DNA transfer in both stable and transient transfections of eukaryotic cells and is ideal for high-throughput transfections in a multi-well plate format. The unique composition is compatible with both serum-containing and serum-free media, making media changes unnecessary during transfection experiments. Genejuice is a superior alternative to a wide variety of other techniques including calcium phosphate coprecipitation, electroporation, microinjection, biolistic particle delivery, lipofection, and complex formation with DEAE-dextran. The 1 ml size provides enough reagent to perform up to 500 transfections in standard 35 mm plates.
Cell lines transfected with GeneJuice® Transfection Reagent
Cell Lines: | Primary Cells: | ||||
---|---|---|---|---|---|
10T1/2 293T 3T3 NIH 3T3 Swiss 3T3-L1 A204 A431 A549 alpha TC1-6 AR 42J As4.1 AtT-20 B50 BC-1 BC-2 BC3 BCBL BHK-21 C3H/10T1/2 C6 C2C12 | Caco-2 Caki-1 Calpan-1 Calu-1 Calu-6 CCL-131 CFPAC-1 Chang Liver CHO CHO-7 CHO-IR CHO-K1 COS-1 COS-7 CS-1 CV-1 Daudi DDTI MF-2 DT40 ECV304 EL4 | ES-E14TG2a EVSCC17M H9c2 HCT-116 HEK293 HeLa HeLa B HeLa T4 Hep 3B2.1-7 HepG2 Hepa 1-6 Ht-29 HTB-37 HTB-45 Huh-7 HUVEC IC21 IEC-6 JEG-3 Jurkat KB | L57-3-11 L-6 L-929 MA-10 McA-RH7777 MCF-7 MCF-10-2A MDCK Melanocyte MG-63 Neuro 2A Neuroblastoma NRK NT2/D1 OV-1063 OVCAR3 P4 P19 PC12 PA317 PAM212 | PS-1 R2C RAW 264.7 RBL-2H3 RMP-41 SAOS-2 SC-1 Schneider line2 SK-N-MC SK-N-SH SKOV3 STO SW-480 SW-837 T3M4 TM4 U937 UCD Vero | Aortic smooth muscle cells Astrocytes Angioblasts Chondrocytes Chromaffin cells Epithelial cells: mammary prostate tracheal Fibroblasts Keratinocytes |
특징 및 장점
- Highly efficient DNA transfer for both stable and transient transfections
- Compatibility with both serum-containing and serum-free media
- Simple protocolno need for media changes
- Ideal for high-throughput transfection in a multi-well plate format
- Ideal for retrovirus production for T-cell transduction
- Works for a multitude of cell types with minimal optimization
- Provides higher transfection efficiency and lower cytotoxicity than reagents from other suppliers
- The 1 ml size provides enough reagent to perform up to 500 transfections in standard 35 mm plates
경고
제조 메모
기타 정보
법적 정보
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2
Storage Class Code
3 - Flammable liquids
WGK
WGK 1
Flash Point (°F)
67.1 °F - Information taken from reference works and the literature.
Flash Point (°C)
19.5 °C - Information taken from reference works and the literature.
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
관련 콘텐츠
Major technological advances have made the production of monoclonal antibodies quicker and more efficient. There are three established platforms for antibody discovery. We offer reagents for production of monoclonal antibody libraries using each of these techniques.
최근 기술 분야에서 중대한 발전들이 이뤄지면서 단클론 항체 생성의 속도와 효율성이 더욱 높아졌습니다. 항체 발견을 위해 확립된 플랫폼은 3가지가 있습니다. Merck는 이 기법들을 각각 이용해서 단클론 항체 라이브러리를 생성하는 데 필요한 시약을 제공합니다.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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