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Merck
모든 사진(6)

문서

70967

Sigma-Aldrich

GeneJuice® Transfection Reagent

Non-lipid based chemical transfection reagent optimized for maximum transfection efficiency, ease-of-use, and minimal cytotoxicity on a wide variety of mammalian cells.

동의어(들):

GeneJuice Transfection, Transfection Reagent

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About This Item

UNSPSC 코드:
41106502
NACRES:
NA.54

Quality Level

형태

liquid

제조업체/상표

Novagen®

저장 조건

OK to freeze

기술

transfection: suitable

배송 상태

wet ice

저장 온도

2-8°C

일반 설명

""I use GeneJuice because it′s ease-of-use, low toxicity and wide range of target cells""
Stuart Rulten. Research Fellow. University of Sussex

""Since we have been working with GeneJuice, we have saved a lot of time and money!""
Dr. Andrea Kress, Institute of Clinical and Molecular Virology, Erlangen, Germany

""I find that Genejuice works for a multitude of cell types with minimal optimization""
Caitriona Marie Lyons. PhD Student. University College Cork, Ireland

Transfection is the process by which nucleic acids are introduced into mammalian cells. GeneJuice Transfection Reagent is a proprietary formulation optimized for maximal transfection efficiency, ease of use, and minimal cytotoxicity for mammalian cells. Whereas many available transfection reagents are based on cationic lipid formulation, GeneJuice Transfection Reagent is composed of a nontoxic cellular protein and a small amount of a novel polyamine. GeneJuice Transfection Reagent enables highly efficient DNA transfer in both stable and transient transfections of eukaryotic cells and is ideal for high-throughput transfections in a multi-well plate format. The unique composition is compatible with both serum-containing and serum-free media, making media changes unnecessary during transfection experiments. Genejuice is a superior alternative to a wide variety of other techniques including calcium phosphate coprecipitation, electroporation, microinjection, biolistic particle delivery, lipofection, and complex formation with DEAE-dextran. The 1 ml size provides enough reagent to perform up to 500 transfections in standard 35 mm plates.


Cell lines transfected with GeneJuice® Transfection Reagent
Cell Lines:Primary Cells:
10T1/2

293T

3T3 NIH

3T3 Swiss

3T3-L1

A204

A431

A549

alpha TC1-6

AR 42J

As4.1

AtT-20

B50

BC-1

BC-2

BC3

BCBL

BHK-21

C3H/10T1/2

C6

C2C12
Caco-2

Caki-1

Calpan-1

Calu-1

Calu-6

CCL-131

CFPAC-1

Chang Liver

CHO

CHO-7

CHO-IR

CHO-K1

COS-1

COS-7

CS-1

CV-1

Daudi

DDTI MF-2

DT40

ECV304

EL4
ES-E14TG2a

EVSCC17M

H9c2

HCT-116

HEK293

HeLa

HeLa B

HeLa T4

Hep 3B2.1-7

HepG2

Hepa 1-6

Ht-29

HTB-37

HTB-45

Huh-7

HUVEC

IC21

IEC-6

JEG-3

Jurkat

KB
L57-3-11

L-6

L-929

MA-10

McA-RH7777

MCF-7

MCF-10-2A

MDCK

Melanocyte

MG-63

Neuro 2A

Neuroblastoma

NRK

NT2/D1

OV-1063

OVCAR3

P4

P19

PC12

PA317

PAM212
PS-1

R2C

RAW 264.7

RBL-2H3

RMP-41

SAOS-2

SC-1

Schneider line2

SK-N-MC

SK-N-SH

SKOV3

STO

SW-480

SW-837

T3M4

TM4

U937

UCD

Vero

Aortic smooth muscle cells

Astrocytes

Angioblasts

Chondrocytes

Chromaffin cells

Epithelial cells:

mammary

prostate

tracheal

Fibroblasts

Keratinocytes

Non-lipid based chemical transfection reagent optimized for maximum transfection efficiency, ease-of-use, and minimal cytotoxicity on a wide variety of mammalian cells.

특징 및 장점

  • Highly efficient DNA transfer for both stable and transient transfections
  • Compatibility with both serum-containing and serum-free media
  • Simple protocolno need for media changes
  • Ideal for high-throughput transfection in a multi-well plate format
  • Ideal for retrovirus production for T-cell transduction
  • Works for a multitude of cell types with minimal optimization
  • Provides higher transfection efficiency and lower cytotoxicity than reagents from other suppliers
  • The 1 ml size provides enough reagent to perform up to 500 transfections in standard 35 mm plates

경고

Toxicity: Flammable (J)

제조 메모

For protocol, please click (here)

기타 정보

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

법적 정보

GeneJuice® is a registered trademark of EMD Chemicals Inc.
GENEJUICE is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

픽토그램

FlameExclamation mark

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point (°F)

67.1 °F - Information taken from reference works and the literature.

Flash Point (°C)

19.5 °C - Information taken from reference works and the literature.


시험 성적서(COA)

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Sharon Eisenberg et al.
Molecular and cellular biology, 31(19), 3938-3952 (2011-08-03)
The trafficking, membrane localization, and lipid raft association of Ras proteins, which are crucial oncogenic mediators, dictate their isoform-specific biological responses. Accordingly, their spatiotemporal dynamics are tightly regulated. While extensively studied for H- and K-Ras, such information on N-Ras, an
Linda Brunotte et al.
The Journal of biological chemistry, 286(44), 38748-38756 (2011-09-16)
The nucleoprotein (NP) of Lassa virus (LASV) strain AV was expressed in a recombinant baculovirus system. The crystal structure of full-length NP was solved at a resolution of 2.45 Å. The overall fold corresponds to that of NP of LASV
Mark G Frost et al.
Bioscience reports, 40(6) (2020-05-19)
Fanconi Anemia (FA) is a rare genetic disorder characterized by developmental defects, bone marrow failure and high predisposition to cancer. The FA DNA repair pathway is required in humans to coordinate repair of DNA interstrand cross-links. The central event in
Karel De Gendt et al.
Biology of reproduction, 85(5), 934-945 (2011-07-08)
Our previous analysis of Sertoli cell androgen receptor (AR) knockout (SCARKO) mice revealed that several cytoskeletal components are a potential target of androgen action. Here, we found that one of these components, the beta-tubulin isotype Tubb3, is differentially regulated in
Natasha C Lucki et al.
The Journal of biological chemistry, 286(22), 19399-19409 (2011-04-16)
Sphingolipid metabolites, such as ceramide (Cer), sphingosine (SPH), and sphingosine 1-phosphate (S1P), contribute to multiple aspects of carcinogenesis including cell proliferation, migration, angiogenesis, and tumor resistance. The cellular balance between Cer and S1P levels, for example, is an important determinant

관련 콘텐츠

Major technological advances have made the production of monoclonal antibodies quicker and more efficient. There are three established platforms for antibody discovery. We offer reagents for production of monoclonal antibody libraries using each of these techniques.

최근 기술 분야에서 중대한 발전들이 이뤄지면서 단클론 항체 생성의 속도와 효율성이 더욱 높아졌습니다. 항체 발견을 위해 확립된 플랫폼은 3가지가 있습니다. Merck는 이 기법들을 각각 이용해서 단클론 항체 라이브러리를 생성하는 데 필요한 시약을 제공합니다.

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