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BugBuster^® Master Mix

• 동의어(들): BugBuster^® Protein Extraction Reagent with Benzonase^® Nuclease and rLysozyme solution, Cell Lysis Master Mix
• UNSPSC 코드: 41106511
• NACRES: NA.32

속성

• 형태: liquid
• Quality Level: 200
• 제조업체/상표: Novagen^®
• 저장 조건: OK to freeze
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

The BugBuster^® Master Mix enables efficient protein purification and other related applications. It replaces the need for mechanical methods like the French press or sonication. The two available package sizes contain sufficient reagents for extracting proteins from either 20 grams or 100 grams of cell paste.

애플리케이션

BugBuster^® Master Mix has been used:

• to resuspend and lyse the cell pellets during protein purification
• to extract proteins for immunoblotting
• as a bacterial lysis buffer for the preparation of inclusion bodies

    

생화학적/생리학적 작용

BugBuster^® Protein Extraction Reagent is specifically designed to gently disrupt the cell wall of E. coli, allowing the release of soluble proteins. This unique formulation utilizes a detergent mix that can effectively perforate the cell wall without denaturing the soluble proteins. By combining BugBuster Reagent, rLysozyme solution, and Benzonase Nuclease, our BugBuster Master Mix enhances protein extraction efficiency and simplifies downstream processing of protein extracts.

특징 및 장점

• Efficient lysis of Gram-positive and Gram-negative bacteria
• Maximum recovery of active, soluble protein
• Capable of cell wall perforation without denaturing soluble protein
• Useful for the preparation of high purity inclusion bodies
• Compatible with protease and phosphatase inhibitor cocktail

• Offers a convenient, fast, and cost-effective solution for releasing the expressed target protein.
• The Master Mix eliminates the need to dilute or perform separate addition steps.

경고

Toxicity: Standard Handling (A)

법적 정보

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable