추천 제품
![(4R,4′R,5S,5′S)-2,2′-Cyclopropylidenebis[4,5-dihydro-4,5-diphenyloxazole] ≥95%](/deepweb/assets/sigmaaldrich/product/structures/104/825/126fc1d0-0369-45da-95f0-5f745b5cf8fa/640/126fc1d0-0369-45da-95f0-5f745b5cf8fa.png)
생물학적 소스
rabbit
Quality Level
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성(상동성에 의해 예측)
all
제조업체/상표
Chemicon®
기술
ELISA: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
배송 상태
wet ice
타겟 번역 후 변형
phosphorylation (pThr)
일반 설명
Protein phosphorylation and dephosphorylation are basic mechanisms for the modification of protein function in eukaryotic cells. Phosphorylation is a rare post-translational event in normal tissue, however, the abundance of phosphorylated cellular proteins increases several fold following various activation processes which are mediated through phosphotyrosine, phosphoserine or phosphothreonine (p-tyr/p-ser/p-thr). Many signal transduction pathways, such as the EGF, PDGF and insulin receptor systems, contain tyr/ser/thr kinase which phosphorylate specific tyr/ser/thr residues upon binding of ligands to their receptors. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate these phosphorylation associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated tyr/ser/thr.
특이성
Anti-phosphothreonine is species independent
By direct competitive ELISA methodology (antigen capture) the specificity of this antibody has been determined to be phosphothreonine. Binding of Ly-pT-Gly-HRP (0.2μg/100μL/well) to an anti-phosphothreonine coated microplate (0.5μg/100μL/well) is inhibited by 95% with the addition of 2-10 μg/mL anti-phosphothreonine. No inhibition is evident by inhibition of phosphoserine, phosphotyrosine, or threonine at concentrations of up to 1 mg/mL. Antibody AB1607 recognizes phosphothreonine, peptidyl-phosphothreonine, and threonine-phosphorylated proteins.
면역원
Keyhole Limpet Hemocyanin (KLH) – conjugated phosphothreonine conjugates and Phosvitin.
애플리케이션
Anti-Phosphothreonine Antibody is an antibody against Phosphothreonine for use in ELISA, IH(P), IP & WB.
ELISA (kinase assay):
A 0.5 μg/mL concentration of a previous lot was used in ELISA.
Immunoprecipitation:
A previous lot of this antibody was used in IP.
Immunohistochemistry (paraffin):
Representative image from a previous lot.
Optimal working dilutions must be determined by end user.
A 0.5 μg/mL concentration of a previous lot was used in ELISA.
Immunoprecipitation:
A previous lot of this antibody was used in IP.
Immunohistochemistry (paraffin):
Representative image from a previous lot.
Optimal working dilutions must be determined by end user.
Research Category
Signaling
Signaling
Research Sub Category
General Post-translation Modification
General Post-translation Modification
품질
Routinely evaluated by Western Blot on Calyculin/Okadaic Acid treated HEK293 lysate.
Western Blot Analysis: 1:500 dilution of this lot detected PHOSPHOTHREONINE on 10 μg of Calyculin/Okadaic Acid treated HEK293 lysate.
Western Blot Analysis: 1:500 dilution of this lot detected PHOSPHOTHREONINE on 10 μg of Calyculin/Okadaic Acid treated HEK293 lysate.
물리적 형태
ImmunoAffinity Purified
Purified rabbit polyclonal in PBS buffer, containing 50% Glycerol, 0.01% NaN3.
저장 및 안정성
Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
분석 메모
Control
Mouse brain lysate.
Mouse brain lysate.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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