추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
affinity purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성
mouse, rat
제조업체/상표
Chemicon®
기술
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
mouse ... Drd3(13490)
rat ... Drd3(29238)
특이성
Specific for Dopamine Receptor D3. Rat Dopamine D3 receptor is a 446 aa, G-protein coupled and transmembrane receptor protein (Giros et al. 1990, 1991; Schmauss et al. 1993; Liu et al. 1994; Fu et al. 1995; Sokoloff et al. 1990; Livingstone et al. 1992). The immunogen peptide shows no significant homology with other dopamine receptors (D1, D2, D4 or D5).
SPECIES REACTIVITIES: The immunogen peptide shows sequence homology of 88% with mouse and 63% with human/monkey (12/19 aa) D3 receptors (Giros et al. 1990, 1991; Schmauss et al. 1993; Liu et al. 1994; Fu et al. 1995; Sokoloff et al. 1990; Livingstone et al. 1992). Antibody cross-reactivity with various species is not known.
SPECIES REACTIVITIES: The immunogen peptide shows sequence homology of 88% with mouse and 63% with human/monkey (12/19 aa) D3 receptors (Giros et al. 1990, 1991; Schmauss et al. 1993; Liu et al. 1994; Fu et al. 1995; Sokoloff et al. 1990; Livingstone et al. 1992). Antibody cross-reactivity with various species is not known.
면역원
A 19 amino acid peptide sequence from the rat D3 receptor within the 3rd cytoplasmic domain.
Epitope: cytoplasmic domain
애플리케이션
Anti-Dopamine D3 Receptor Antibody, cytoplasmic domain detects level of Dopamine D3 Receptor & has been published & validated for use in ELISA, IH & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
Western blot: 1-10 μg/mL (Chemiluminescence technique; 45-50 kDa) Approximately 100-400 μg of membrane protein/lane should be loaded to visualize the receptor band. In some systems 70kDa bands may also be present.
Immunohistochemistry: suggested working concentration is 2-10 μg/mL. Suggested fixative is 4% PFA, fixation 10-15′, permeabilized with 0.3-0.5% triton X-100 for 10-15′ (tissue, less time for cels), primary antibody dilution is 1:100 for direct fluorescence . {Coronas, V, et al. J Neurochemistry 91:1292-1301, 2004},
ELISA: 0.5-1.0 μg/mL (1 μg/mL immunogen peptide (Catalog Number AG229)/well)
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: suggested working concentration is 2-10 μg/mL. Suggested fixative is 4% PFA, fixation 10-15′, permeabilized with 0.3-0.5% triton X-100 for 10-15′ (tissue, less time for cels), primary antibody dilution is 1:100 for direct fluorescence . {Coronas, V, et al. J Neurochemistry 91:1292-1301, 2004},
ELISA: 0.5-1.0 μg/mL (1 μg/mL immunogen peptide (Catalog Number AG229)/well)
Optimal working dilutions must be determined by the end user.
물리적 형태
Affinity purified immunoglobulin. Liquid in PBS with 0.1% BSA.
저장 및 안정성
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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