AB1870

Anti-Osteopontin Antibody

serum, Chemicon^®

• 동의어(들): Uropontin, Bone sialoprotein 1, SPP1/CALPHA1 fusion, Urinary stone protein, bone sialoprotein I, osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein, secreted phosphoprotein 1, secreted phosphoprotein 1 (osteopontin, bone sialop
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: serum
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 종 반응성: mouse, rat, human
• 제조업체/상표: Chemicon^®
• 기술: ELISA: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• NCBI 수납 번호: NP_000573
• UniProt 수납 번호: P10451
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... SPP1(6696)

설명

일반 설명

Osteopontin is a single-chain polypeptide with a molecular weight of approximately 35 kDa. It is a glycoprotein first identified in osteoblasts. It is biosynthesized by a variety of tissue types including preosteoblasts, osteoblasts, osteocytes, extraosseous cells in the inner ear, brain, kidney, deciduum, placenta, odontoblasts, some bone marrow cells, hypertrophic chondrocytes, macrophages, smooth muscle, and endothelial cells. Osteopontin is overexpressed in a variety of cancers, including lung cancer, breast cancer, colorectal cancer, stomach cancer, ovarian cancer, melanoma and mesothelioma. It may contribute to kidney stone formation and both glomerulonephritis and tubulointerstitial nephritis and is also found in atheromatous plaques within arteries. Research has implicated osteopontin in excessive scar-forming and a gel has been developed to inhibit its effect.

특이성

Expected to cross-react with rat.

Human osteopontin. Does not cross react with osteonectin or human bone sialoprotein II.

면역원

Synthetic peptide corresponding to amino acids 75-90 of human osteopontin (SwissProt Accession # P10451; PSKSNESHDHMDDM DD).

애플리케이션

Immunohistochemistry:
A previous lot of this antibodu was used at a 1:1500 dilution on paraffin-embedded tissue sections.

ELISA:
A previous lot of this antibody was used at a 1:1000 dilution.

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Osteobiology

This Anti-Osteopontin Antibody is validated for use in ELISA, IH, IH(P), WB for the detection of Osteopontin.

품질

Routinely evaluated by Western Blot on RAW 264.7 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected osteopontin on 10 μg of RAW 264.7 lysates.

표적 설명

~35 kDa

물리적 형태

Rabbit polyclonal antiserum in buffer containing no preservatives.

Unpurified

저장 및 안정성

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

분석 메모

Control
POSITIVE CONTROL: Human urine (2-5 µg/mL normal concentration).

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable