추천 제품
제품명
Anti-GAPDH Antibody, from chicken, purified by affinity chromatography
생물학적 소스
chicken
Quality Level
항체 형태
affinity isolated antibody
항체 생산 유형
primary antibodies
클론
polyclonal
정제법
affinity chromatography
종 반응성
human, mouse, horse, rat
종 반응성(상동성에 의해 예측)
canine (based on 100% sequence homology), equine (based on 100% sequence homology)
포장
antibody small pack of 25 μg
기술
western blot: suitable
NCBI 수납 번호
UniProt 수납 번호
배송 상태
ambient
wet ice
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... GAPDH(2597)
mouse ... Gapdh(14433)
일반 설명
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in reasonably high levels in almost all tissues. As a ′house-keeping′ enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimer′s and Huntington′s disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP).
특이성
This antibody recognizes GAPDH.
면역원
Epitope: WARS interacting region
KLH-conjugated linear peptide corresponding to the WARS interacting region of human GAPDH.
애플리케이션
Anti-GAPDH Antibody detects level of GAPDH & has been published & validated for use in WB.
Research Category
Metabolism
Metabolism
Western Blot (SNAP ID) Analysis: 0.125 µg/ml from a previous lot detected GAPDH on 10 µg of NIH/3T3 and PC12 cell lysates.
품질
Evaluated by Western Blot in HEK293 and PC12 cell lysate.
Western Blot Analysis: 0.0625 µg/ml of this antibody detected GAPDH on 10 µg of HEK293 and PC12 cell lysate.
Western Blot Analysis: 0.0625 µg/ml of this antibody detected GAPDH on 10 µg of HEK293 and PC12 cell lysate.
표적 설명
~ 38 kDa
물리적 형태
Affinity purified
Purified chicken polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
저장 및 안정성
Stable for 1 year at 2-8°C from date of receipt.
분석 메모
Control
HEK293 cell lysate
HEK293 cell lysate
기타 정보
Concentration: Please refer to lot specific datasheet.
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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