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Anti-GAPDH Antibody, clone 10B13, ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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EC:, Glyceraldehyde-3-phosphate dehydrogenase, Peptidyl-cysteine S-nitrosylase GAPDH

biological source

rabbit (recombinant)

Quality Level


expressed in HEK 293 cells



antibody form

purified antibody

antibody product type

primary antibodies


10B13, monoclonal
recombinant monoclonal


10B13 Clone

product line

ZooMAb® learn more



mol wt

calculated mol wt 36.05 kDa
observed mol wt ~36 kDa

species reactivity



antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation


immunofluorescence: suitable using 1:100
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using 1:100
western blot: suitable using 1:1,000



UniProt accession no.

greener alternative category

shipped in


storage temp.


target post-translational modification


Gene Information

human ... GAPDH(2597)

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Gene Information

human ... GAPDH(2597)

Gene Information

human ... ATG9A;APG9L1(79065)

Gene Information

rat ... Slc17a7;Bnpi;Vglut1(116638)

Gene Information

human ... SMAD3;MADH3(4088)

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody


10B13, monoclonal, recombinant monoclonal


5B11, monoclonal, recombinant monoclonal


4O15, monoclonal, recombinant monoclonal


1J11, recombinant monoclonal

biological source

rabbit (recombinant)

biological source

rabbit (recombinant)

biological source

rabbit (recombinant)

biological source

rabbit (recombinant)

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General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.


Clone 10B13 is a ZooMab rabbit recombinant monoclonal antibody that specifically detects human Glyceraldehyde-3-phosphate dehydrogenase and targets an epitope with in the C-terminal half.


GST/His-tagged recombinant fragment corresponding to 190 amino acids from the C-terminal half of human Glyceraldehyde-3-phosphate dehydrogenase (GAPDH).


Anti-GAPDH, clone 10B13 ZooMAb, Cat. No. ZRB374, is a a recombinant Rabbit Monoclonal Antibody that specifically targets Glyceraldehyde-3-phosphate dehydrogenase and ha been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Wetern Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GAPDH in HEK293 cell lysate.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected GAPDH in human testis tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected GAPDH in human testis tissue sections.

Target description

Glyceraldehyde-3-phosphate dehydrogenase (UniProt: P04406; also known as EC:, GAPDH, Peptidyl-cysteine S-nitrosylase GAPDH) is encoded by the GAPDH (also known as GAPD, CDABP0047, OK/SW-cl.1) gene (Gene ID: 2597) in human. GAPDH is considered as a ′house-keeping′ enzyme that catalyzes the synthesis of 1,3-biphosphoglycerate. It is a homotetrameric enzyme with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities. Hence, it plays a role in both glycolysis and nuclear functions. It translocates to the nucleus following S-nitrosylation on cysteine 152 that leads to its interaction with SIAH1, which contains a nuclear localization signal. In the nucleus it participates in transcription, RNA transport, DNA replication and apoptosis. Sulfhydration at cysteine 152 is reported to increase its catalytic activity. Its nuclear functions are attributed to its nitrosylase activity, which mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2, and DNA-PK. Oxidative stress is reported to promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process known as nucleocytoplasmic coagulation. These aggregates are observed in vivo in the affected tissues of patients with Alzheimer disease or alcoholic liver cirrhosis, or in cell cultures during necrosis. Oxidation at methionine 46 plays an essential role in the formation of these insoluble structures. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.


30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids




Not applicable


Not applicable

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Choose sustainable options for your research with greener alternative ZooMAb® recombinant antibodies. These BSA-free, carrier-free antibodies are shipped in low-waste packaging and offer maximum stability without compromising performance.

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