MilliporeSigma
All Photos(3)

Documents

ABS16

Sigma-Aldrich

Anti-GAPDH Antibody

from rabbit, purified by affinity chromatography

Sign Into View Organizational & Contract Pricing

Synonym(s):
glyceraldehyde 3-phosphate dehydrogenase, aging-associated gene 9 protein, glyceraldehyde-3-phosphate dehydrogenase, GAPDH, G3PDH, GAPD, OK/SW-cl.12, MGC88685, G3PD, EC 1.2.1.12
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human, rat

species reactivity (predicted by homology)

guinea pig (based on 100% sequence homology), bovine (based on 100% sequence homology)

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GAPDH(2597)
mouse ... Gapdh(14433)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
AB2302MAB374SAB2500450
vibrant-m

ABS16

Anti-GAPDH Antibody

vibrant-m

AB2302

Anti-GAPDH Antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

biological source

rabbit

biological source

chicken

biological source

mouse

biological source

goat

Gene Information

human ... GAPDH(2597)
mouse ... Gapdh(14433)

Gene Information

human ... GAPDH(2597)
mouse ... Gapdh(14433)

Gene Information

human ... GAPDH(2597)
mouse ... Gapdh(14433)

Gene Information

human ... GAPDH(2597)

species reactivity

mouse, human, rat

species reactivity

human, mouse, horse, rat

species reactivity

human, feline, pig, mouse, rabbit, fish, canine, rat

species reactivity

mouse, human, rat

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

ELISA: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunofluorescence: suitable, immunohistochemistry: suitable, indirect ELISA: suitable, western blot: suitable

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is an ubiquitous glycolytic enzyme present in moderately high levels in almost all tissues. As a ′house-keeping′ enzyme, it catalyzes the synthesis of 1,3-biphosphoglycerate, a "high energy" intermediate used for the synthesis of ATP. Besides its cytoplasmic action in metabolism it is also involved in the initial stages of apoptosis or oxidative stress response where GAPDH is translocated to the nucleus. Such actions may reflect the role of GAPDH in DNA repair or as one nuclear carrier for apoptotic molecules. GAPDH has also been found to bind specifically to proteins implicated in the pathogenesis of a variety of neurodegenerative disorders including the beta-amyloid precursor protein and the huntingtin protein where decreased function of GAPDH is associated with Alzheimer′s and Huntington′s disease fibroblasts. GAPDH has also been identified as a potential target for nitric oxide (NO)-mediated cellular toxicity. The complete and functional enzyme is a tetramer with each of four identical subunits occupying the vertex of a tetrahedron. Binding domains also include one principally interacting with NAD+ and another interacting with glyceraldehyde 3-phosphate (GAP).

Specificity

This antibody recognizes GAPDH at the C-terminus.

Immunogen

Epitope: C-terminus
GST-tagged recombinant protein corresponding to the C-terminus of human GAPDH.

Application

Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody is popular affinity purified polyclonal antibody. This antibody is supported by peer reviewed publications and reliably detects Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) is validated for use in WB.
Research Category
Signaling

Quality

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected GAPDH on 10 µg of HEK293 cell lysate.

Target description

~36kDa observed

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HEK293 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 1

1 of 1

Mitochondrial peroxiredoxin 3 regulates sensory cell survival in the cochlea.
Chen, FQ; Zheng, HW; Schacht, J; Sha, SH
Testing null
Takeru Sugihara et al.
British journal of cancer, 114(9), 1012-1018 (2016-03-24)
Recent Drosophila studies showed that Discs-large (Dlg) is critical for regulation of cell polarity and tissue architecture. We investigated the possibility that loss of the human homologue of Drosophila Dlg (DLG1) is involved in endometrial carcinogenesis. We analysed DLG1 expression
Jun Chen et al.
Journal of the Association for Research in Otolaryngology : JARO, 17(4), 289-302 (2016-04-21)
Loss of auditory sensory hair cells is the major pathological feature of noise-induced hearing loss (NIHL). Currently, no established clinical therapies for prevention or amelioration of NIHL are available. The absence of treatments is due to our lack of a
Jean Frédéric Boyer et al.
Arthritis research & therapy, 18, 56-56 (2015-01-01)
Anti TNF drugs have been widely used in rheumatoid arthritis (RA) but only 70 to 80 % of patients respond to this therapy. Exploring the mode of action of anti-TNF drugs remains important in order to improve the efficiency of the
Jing Lu et al.
Science China. Life sciences, 57(4), 422-431 (2014-03-20)
Although tau is mainly known as an axonal microtubule-associated protein, many studies indicate that it is not restricted to this subcellular compartment. Assessing tau's subcellular distribution, however, is not trivial as is evident from transgenic mouse studies. When human tau

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service