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Merck

AB3738-I

Anti-Beta Lactamase

from rabbit, purified by affinity chromatography

동의어(들):

Beta-lactamase TEM, IRT-4, Penicillinase, TEM-1, TEM-16/CAZ-7, TEM-2, TEM-24/CAZ-6, TEM-3, TEM-4, TEM-5, TEM-6, TEM-8/CAZ-2

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
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제품 이름

Anti-Beta Lactamase, from rabbit, purified by affinity chromatography

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

E. coli

species reactivity (predicted by homology)

all

technique(s)

western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Quality Level

Analysis Note

Evaluated by Western Blotting in lasate from beta-lactamase-transformed E.coli.

Western Blotting Analysis: A 1:100,000 dilution of this antibody detected GST-tagged full-length E. coli beta-lactamase in 0.5 µg of lysate from transformed E. coli.

Application

Detect beta-lactamase using this rabbit Anti-Beta Lactamase polyclonal antibody, Cat. No. AB3738-I, validated for use in Western Blotting.

Biochem/physiol Actions

This polyclonal antibody detects E. coli beta-lactamase.

General description

31.52/28.91 kDa (with/without signal peptide a.a. 1-23) calculated.
Beta-lactamase TEM (EC 3.5.2.6; UniProt P62593; also known as IRT-4, Penicillinase, TEM-1, TEM-16/CAZ-7, TEM-2, TEM-24/CAZ-6, TEM-3, TEM-4, TEM-5, TEM-6, TEM-8/CAZ-2) is encoded by the bla (also known as blaT-3, blaT-4, blaT-5, blaT-6) gene (Gene ID 3244915) in E. coli. TEM-1 belongs to the class-A beta-lactamase family. Beta-lactamases produced by bacteria provide resistance to beta-lactam antibiotics, such as penicillins and cephamycins by breaking open the beta-lactam ring. Over 200 beta-lactamases have been reported that are classified into four main groups (A, B, C, and D) and eight subgroups according to their functional and structural characteristics. The TEM-1 is predominant plasmid-mediated beta-lactamase of gram-negative rods. Six different nucleotide sequences, called blaTEM-1a, blaTEM-1b, blaTEM-1c, blaTEM-1d, blaTEM-1e, and blaTEM-1f, have been described to codify the same TEM-1 beta-lactamase. Ref.: Briñas, L., et al. (2002). Antimicrob. Agents Chemother. 46, 103156-103163.

Immunogen

GST-tagged full-length E. coli beta-lactamase, including the signal peptide sequence.

Other Notes

Concentration: Please refer to lot specific datasheet.
Replaces: AB3738

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저장 등급

12 - Non Combustible Liquids

wgk

WGK 1


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문서 라이브러리 방문

Nils Peiter et al.
Life science alliance, 6(1) (2022-11-01)
Cotranslational insertion of selenocysteine (Sec) proceeds by recoding UGA to a sense codon. This recoding is governed by the Sec insertion sequence (SECIS) element, an RNA structure on the mRNA, but size, location, structure determinants, and mechanism differ for Bacteria

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