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Merck
모든 사진(1)

주요 문서

AB5972

Sigma-Aldrich

Anti-Dishevelled-2 Antibody

Chemicon®, from rabbit

동의어(들):

DVL2, DSH Homolog 2

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

rabbit

Quality Level

항체 형태

purified immunoglobulin (Partially purified)

항체 생산 유형

primary antibodies

클론

polyclonal

종 반응성

human, mouse

제조업체/상표

Chemicon®

기술

immunohistochemistry: suitable
western blot: suitable

입력

sample type hematopoietic stem cell(s)
sample type neural stem cell(s)

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... DVL2(1856)
mouse ... Dvl2(13543)

일반 설명

DVL2 is a member of multigene family that also contains DVL1 and DVL3. The vertebrate dishevelled proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. DVL2 encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein.

특이성

Disheveiled-2 protein (dvl-2). By Western blot the antibody shows no cross reactivity with recombinant disheveiled-1 (dvl-1) or disheveiled-3 (dvl-3) protein. Pre-adsorption of the antibody with the immunogen peptide completely abolishes its reactivity by Western blot against disheveiled-2 protein.

면역원

Synthetic peptide corresponding to amino acids 600-614 of human and mouse disheveiled-2 protein.

애플리케이션

Detect Dishevelled-2 using this Anti-Dishevelled-2 Antibody validated for use in WB, IH.
Research Category
Stem Cell Research
Research Sub Category
Hematopoietic Stem Cells

Neural Stem Cells
Western blot: 1:1,000 using ECL. The antibody reacts with a protein of ~95kDa. Faint reactivity was seen in the dvl-1 and dvl-3 HEK293 cell lysates suggesting there may be endogenous dvl-2 present.

Immunohistochemistry: 1:1,000 using the avidin-biotin system.

Dilute antibody in 50 mM PBS, pH 7.4 containing 1.5% sodium chloride and 1% normal animal serum (same species as the host of the secondary antibody).

Optimal working dilutions must be determined by end user.

표적 설명

80 kDa

물리적 형태

Format: Purified
Partially purified immunoglobulin. Liquid in PBS, pH 7.4, containing 0.01M sodium azide.

저장 및 안정성

Maintain at -20°C in undiluted aliquots (not less than 10 μL) for up to 6 months. Avoid repeated freeze/thaw cycles. The use of high quality antiserum-grade plastic or glass is recommended.

분석 메모

Control
SK-N-SH (human neuroblastoma) whole cell lysates, NIH 3T3 whole cell lysate, BT-20 (human mammary gland) cell lysate, FP (mouse embryonal carcinoma

testicular teratoma) cell lysate

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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