AB6001

Anti-MMP-9 Antibody, NT

from rabbit, purified by affinity chromatography

• 동의어(들): 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, macrophage gelatinase, matrix metallopeptidase 9, matrix metalloproteinase 9, type V collagenase
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: canine, human
• 종 반응성(상동성에 의해 예측): primate (based on 100% sequence homology), pig (based on 100% sequence homology), dog (based on 100% sequence homology), rat (based on 100% sequence homology), horse (based on 100% sequence homology), monkey (based on 100% sequence homology)
• 기술: western blot: suitable
• NCBI 수납 번호: NP_004985.2
• UniProt 수납 번호: P14780
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... MMP9(4318)

설명

일반 설명

All cells within tissues are surrounded by an extracellular matrix (ECM) giving the tissues shape and structure. The ECM is constantly being remodeled and constant communication is maintained between cells through this matrix. Secreted proteins, termed matrix metalloproteinases (MMPs), are involved in the modulation of cell matrix interactions. MMPs are Zn2+ binding endopeptidases that degrade various components of the ECM. MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion. These enzymes are very potent when active, and are associated with extracellular space inhibitors called TIMPs (tissue inhibitors of matrix metalloproteinases).

MMP9, also known as gelatinase B, is a secreted enzyme which degrades the interstitial collagens, types I, II, and III and is produced by normal alveolar macrophages and granulocytes.

특이성

The antibody recognizes human MMP-9. It does not cross react with MMP-1 or MMP-2.

면역원

Epitope: N-terminus

KLH-conjugated synthetic peptide corresponding to the N-terminus of MMP-9.

애플리케이션

Anti-MMP-9 Antibody, N-terminus is an antibody against MMP-9 for use in WB.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

품질

Evaluated on a representative lot by Western blot using an HL-60 cell lysate supplemented with a PMA conditioned media.

Western Blot Analysis: 0.5 µg/ml of this antibody detected MMP-9 on 10 µg of HL-60 plus PMA conditioned media.

표적 설명

92kDa (pro-form) and 88kDa (active form)

결합

Replaces: AB805

물리적 형태

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
HL-60 plus PMA conditioned media

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable