ABS233

Anti-PI3 Kinase p85 Antibody, N-SH2 domain

from rabbit, purified by affinity chromatography

• 동의어(들): Phosphatidylinositol 3-kinase regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase subunit p85-al
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human, rat
• 종 반응성(상동성에 의해 예측): mouse (based on 100% sequence homology), monkey (based on 100% sequence homology), bovine (based on 100% sequence homology)
• 기술: immunoprecipitation (IP): suitable; western blot: suitable
• NCBI 수납 번호: NP_037137
• UniProt 수납 번호: Q63787
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: bovine ... Pik3R1(282307) ; human ... PIK3R1(5295) ; mouse ... Pik3R1(18708) ; rat ... Pik3R1(25513) ; rhesus monkey ... Pik3R1(698996)

설명

일반 설명

Phosphatidylinositol 3-kinase regulatory subunit alpha (UniProt Q63787; also known as Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PtdIns-3-kinase regulatory subunit p85-alpha, PI3-kinase subunit p85-alpha, PI3K regulatory subunit alpha, PtdIns-3-kinase regulatory subunit alpha) is encoded by the Pik3r1 gene (Gene ID 25513) in rat species. PI3K regulatory subunit alpha catalyzes the phosphorylation of PI(4,5)P2 inositol ring at the 3 position to generate PI(3,4,5)P3, a potent second messenger that mediates survival signaling and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Following ligand binding, tyrosine phosphorylation of growth factor receptors creates docking sites for p85 binding (through its SH2 domains), and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Plays a role in insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Mutations in Pik3r1 gene are known to be associated with insulin resistance.

특이성

This polyclonal antibody targets the first SH2 domain region common to human (isoform 1, AS53, p46, p85I), mouse, and rat (isoform p85-alpha, p55-alpha, and p50-alpha) spliced isoforms of PI3 kinase regulatory subunit alpha reported by UniProt (P27986, P26450, Q63787).

면역원

Epitope: First SH2 domain.

GST-tagged recombinant fragment corresponding to the first SH2 domain of rat PI3 kinase regulatory subunit alpha.

애플리케이션

Detect PI3 Kinase regulatory subunit alpha using this rabbit polyclonal Anti-PI3 Kinase p85, N-SH2 domain, Cat. No. ABS233, validated for use in Immunoprecipitation and Western Blotting.

Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated PI3 kinase regulatory subunit alpha isoforms from 200 µg of rat brain tissue lysate.

Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated PI3 kinase regulatory subunit alpha isoforms from 500 µg of Jurkat cell lysate.

Western Blotting Analysis: 2 µg/mL from a representative lot detected PI3 kinase regulatory subunit alpha isoforms in 10 µg of Jurkat cell lysate.

Research Category
Signaling

Research Sub Category
Developmental Signaling

품질

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PI3 kinase regulatory subunit alpha isoforms in 10 µg of rat brain tissue lysate.

표적 설명

~85/50 kDa observed. 83.47/53.49/49.97/84.47 kDa (human isoform 1/AS53/p46/p85I; UniProt P27986), 83.39 kDa (mouse; UniProt P26450), 83.40/53.39/50.00 kDa (rat p85-alpha/p55-alpha/p50-alpha isoforms; UniProt Q63787). Uncharacterized bands may be observed in some lysate(s).

결합

Replaces: 06-496

물리적 형태

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Jurkat cell lysate

기타 정보

Concentration: Please refer to lot specific datasheet.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable