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Merck
모든 사진(1)

주요 문서

IPVH85R

Millipore

Immobilon®-P Membrane

1 roll, 8.5 cm x 10 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

동의어(들):

Immobilon® NOW, Immobilon® narrow roll, Western blotting membrane, blotting membrane, transfer membrane

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About This Item

UNSPSC 코드:
41105339
eCl@ss:
32031613
NACRES:
NB.23

product name

Immobilon®-P Membrane, PVDF, 0.45 m, 8.5 cm x 10 m roll, 0.45 um ( 0.45 micron filter ) pore size Hydrophobic immobilon-P PVDF Transfer Membrane for western blotting using Chemiluminescence, Chromogenic or Radioactive detection methods. Comes in an 8.5 cm x 10 m roll.

material

PVDF membrane
plain filter
white filter

Quality Level

특징

hydrophobic

제조업체/상표

Immobilon®

기술

dot blot: suitable
western blot: suitable

필터 길이 × 너비

8.5 cm × 10 m

공극 크기

0.45 μm pore size

용량

160 μg/cm2 adsorption capacity (insulin)
215 μg/cm2 adsorption capacity (BSA)
294 μg/cm2 adsorption capacity (goat IgG)

호환성

for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Toluidine blue
for use with Transillumination

검출 방법

chemiluminescent
colorimetric
fluorometric
radioactive

배송 상태

ambient

일반 설명

Immobilon®-P transfer membrane is a hydrophobic polyvinylidene fluoride (PVDF) microporous membrane with a nominal pore size of 0.45 micron (μm). It is used for the transfer of proteins from a variety of gel matrices. Immobilon®-P transfer membrane is recommended for most western blotting, especially proteins greater than 20 kDa. This membrane displays improved handling characteristics and staining capabilities, a higher signal-to-noise ratio for enhanced sensitivities, and improved solvent resistance, when compared to nitrocellulose membrane.
Primary Binding Mechanisms: Electrostatic, hydrophobic,
Filter Code: IPVH

애플리케이션

Immobilon®-P Membrane, PVDF, 0.45 μm, 8.5 cm x 10 m roll has been used in western blot analysis and co-immunoprecipitation.

결합

Replaces: INCP00010

법적 정보

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서

A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.

프로토콜

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

폴리아크릴아미드 겔 화학의 개요와 mPAGE® TurboMix Bis-Tris 겔 캐스팅 키트를 이용한 폴리아크릴아미드 겔 핸드캐스팅에 대한 상세 지침

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

관련 콘텐츠

Find protein research tools to prepare, isolate, and analyze proteins. Organized by how to extract, protect, purify, enrich, modify, and quantify proteins.

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