추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
3H2, monoclonal
종 반응성
rat, human, mouse
제조업체/상표
Chemicon®
기술
ELISA: suitable
immunocytochemistry: suitable
western blot: suitable
입력
sample type neural stem cell(s)
sample type mesenchymal stem cell(s)
동형
IgG2b
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... BMP4(652)
mouse ... Bmp4(12159)
rat ... Bmp4(25296)
일반 설명
Bone morphogenetic protein 4 (BMP4) is a polypeptide belonging to the TGF-β superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signalling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. If these cells also receive signals from FGF, they will differentiate into the spinal cord; in the absence of FGF the cells become brain tissue.
특이성
Specific for bone morphogenetic protein-4 (BMP-4) by Western blot and ELISA. No cross-reactivity with human BMP-2 or human TGF-β1. The antibody reacts with both human and mouse BMP-4 in both reduced and nonreduced conditions.
Human BMP-4 (accession number P12644) precursor (389 aa, glycosilated) consists of a propeptide (273 aa) and active BMP-4 (116 aa, glycoslyated; MW reduced 17-20 kDa).
Human BMP-4 (accession number P12644) precursor (389 aa, glycosilated) consists of a propeptide (273 aa) and active BMP-4 (116 aa, glycoslyated; MW reduced 17-20 kDa).
면역원
Recombinant mouse BMP-4 (Masuhara, 1995).
애플리케이션
Detect Bone Morphogenetic Protein 4 using this Anti-Bone Morphogenetic Protein 4 Antibody, clone 3H2 validated for use in ELISA, IC & WB.
Immunocytochemistry using osteoinductive cells; i.e., BFP and Saos-2
Western blot using osteoinductive cells; i.e., BFP and Saos-2
Optimal working dilutions must be determined by the end user.
Western blot using osteoinductive cells; i.e., BFP and Saos-2
Optimal working dilutions must be determined by the end user.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Mesenchymal Stem Cells
Mesenchymal Stem Cells
표적 설명
17 kDa
물리적 형태
Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified
저장 및 안정성
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
분석 메모
Control
Mouse brain extract
Mouse brain extract
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Ling Wu et al.
Experimental and therapeutic medicine, 17(4), 2648-2656 (2019-03-25)
Clarifying the mechanisms via which pacemaker- like cells are generated is critical for identifying novel targets for arrhythmia-associated disorders and constructing pacemakers with the ability to adapt to physiological requirements. T-box 18 (Tbx18)+ epicardial progenitor cells (EPCs) have the potential
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Redox biology, 43, 101979-101979 (2021-04-26)
Loss of perivascular adipose tissue (PVAT) impairs endothelial function and enhances atherosclerosis. However, the roles of PVAT thermoregulation in vascular inflammation and the development of atherosclerosis remains unclear. Bone morphogenetic protein 4 (BMP4) transforms white adipocyte to beige adipocyte, while
Birgit Gustafson et al.
Diabetes, 64(5), 1670-1681 (2015-01-22)
The limited expandability of subcutaneous adipose tissue, due to reduced ability to recruit and differentiate new adipocytes, prevents its buffering effect in obesity and is characterized by expanded adipocytes (hypertrophic obesity). Bone morphogenetic protein-4 (BMP4) plays a key role in
Thomas Helbing et al.
The Journal of pathology, 231(1), 105-116 (2013-05-30)
Epithelial injury is a central finding in pulmonary disease and is accompanied by disruption of epithelial barrier function, leading to pulmonary oedema and inflammation. Injured epithelial cells lose their properties and gain mesenchymal characteristics, a phenotypic switch that contributes to
Vivien Jane Coulson-Thomas et al.
The Journal of biological chemistry, 289(36), 25211-25226 (2014-07-24)
Hair follicle (HF) morphogenesis and cycling are a result of intricate autonomous epithelial-mesenchymal interactions. Once the first HF cycle is complete it repeatedly undergoes cyclic transformations. Heparan sulfate (HS) proteoglycans are found on the cell surface and in the extracellular
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