MAB1222

Anti-Tryptase Antibody, Mast Cell, clone G3

clone G3, Chemicon^®, from mouse

• 동의어(들): Mast cell tryptase antibody
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• 클론: G3, monoclonal
• application: FACS; IHC; WB
• 종 반응성: human
• 기술: flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• citations: 60

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 클론: G3, monoclonal
• 종 반응성: human
• 제조업체/상표: Chemicon^®
• 기술: flow cytometry: suitable; immunohistochemistry: suitable; western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NM_003294.3
• UniProt 수납 번호: Q15661
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified

설명

일반 설명

Tryptase is the most abundant secretory granule-derived serine proteinase contained in mast cells. Tryptase has recently been used as a marker for mast cell activation and is involved with allergic response. Tryptase may act as a mitogen for fibroblast lines. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis.

특이성

Recognizes Mast cell tryptase. Will show reactivity to basophils but to a lesser degree.

애플리케이션

Flow Cytometry Analysis: A previous lot of this antibody has been used on fixed cells in FACS using a conjugated secondary antibody. (Sperr, W., et al., 2001).

Optimal working dilutions must be determined by end user.

Research Category
Inflammation & Immunology

Research Sub Category
Inflammation & Autoimmune Mechanisms

This Anti-Tryptase Antibody, Mast Cell, clone G3 is validated for use in FC, IH, WB for the detection of Tryptase.

품질

Evaluated by western blot on human fetal skin lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected tryptase in 10 µg of human fetal skin lysate.

표적 설명

31 kDa

물리적 형태

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
(see product datasheet for specific buffer formulation)

저장 및 안정성

Maintain at 2–8°C for the duration listed on the product datasheet. (See product datasheet for storage conditions)

분석 메모

Control
Mast cells, basophils

Human fetal skin lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable