추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
HLA.ABC-m3, monoclonal
종 반응성
human
제조업체/상표
Chemicon®
기술
flow cytometry: suitable
immunocytochemistry: suitable
동형
IgG2a
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... HLA-B(3106)
관련 카테고리
일반 설명
The previously assigned protein identifier P03989 has been merged into P01889. Full details can be found on the UniProt database.
특이성
This antibody binds strongly to the HLA-B27 antigen. It also binds less strongly to the HLA-B7 antigen. Affinity studies by Scatchard analysis showed that the antibody has a higher affinity for HLA-B27 (9.7x108M-1) than for HLA-B7
(9.5x107M-1).
Cell reactivity:
Flow cytometric analysis of lymphocyte staining for HLA-B27 gave the following results:
Subjects Fluorescence Intensity
Heterozygous HLA-B27+ Strong
Homozygous HLA-B7+ Strong
Heterozygous HLA-B7+ Faint
Non B27, Non B7 Negative
(9.5x107M-1).
Cell reactivity:
Flow cytometric analysis of lymphocyte staining for HLA-B27 gave the following results:
Subjects Fluorescence Intensity
Heterozygous HLA-B27+ Strong
Homozygous HLA-B7+ Strong
Heterozygous HLA-B7+ Faint
Non B27, Non B7 Negative
애플리케이션
This Anti-HLA-B27 Antibody, clone HLA.ABC-m3 is validated for use in FC, IC for the detection of HLA-B27.
This antibody is useful in the investigation of HLA-B27 in diseases such as ankylosing spondylitis and anterior uveitis.
SUGGESTED USAGE DILUTION
1. Flow cytometry and indirect immunofluorescence 1:60 to 1:120
Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
2. Cytotoxicity testing 1:25.
SUGGESTED USAGE DILUTION
1. Flow cytometry and indirect immunofluorescence 1:60 to 1:120
Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.
2. Cytotoxicity testing 1:25.
물리적 형태
Format: Purified
The antibody is supplied in phosphate-buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by flow cytometry.
저장 및 안정성
Store at 2 to 8°C. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Barbara Neerinckx et al.
RMD open, 3(1), e000451-e000451 (2017-09-08)
The strong genetic association between HLA-B27 and ankylosing spondylitis has been known for over 40 years. HLA-B27 positivity is possibly associated with severity of ankylosis. We studied the in vitro and in vivo impact of HLA-B27 in models of chondrogenesis
Shengli Song et al.
Frontiers in immunology, 12, 705140-705140 (2021-07-31)
Antibody-mediated allograft rejection (AMR) causes more kidney transplant failure than any other single cause. AMR is mediated by antibodies recognizing antigens expressed by the graft, and antibodies generated against major histocompatibility complex (MHC) mismatches are especially problematic. Most research directed
Christoffer Dellgren et al.
Journal of immunology (Baltimore, Md. : 1950), 197(12), 4807-4816 (2016-11-09)
HLA class I cell surface expression is crucial for normal immune responses, and variability in HLA expression may influence the course of infections. We have previously shown that classical HLA class I expression on many human cell types is biased
Congjing An et al.
Cancer cell international, 22(1), 184-184 (2022-05-14)
Breast cancer (BC) is one of the most prevalent malignancies among women globally. Emerging evidence indicates that long non-coding RNAs (lncRNAs) are associated with BC carcinogenesis. In the current study, we explored the mechanism by which LINC00662 regulates BC. Quantitative
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