추천 제품
생물학적 소스
hamster (Armenian)
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
2H8, monoclonal
종 반응성
mouse, canine
제조업체/상표
Chemicon®
기술
electron microscopy: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
neutralization: suitable
동형
IgG
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
dog ... Pecam1(610775)
mouse ... Pecam1(18613)
관련 카테고리
일반 설명
CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets.
CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).
CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas).
특이성
React s with the 130-140 kDa protein, PECAM-1 found on mouse monocytes, platelets, granulocytes and endothelial cells. 2H8 effectively inhibits transmigration of activated polymophonuclear cells and monocytes across the endothelium. In a mouse model for acute peritonis - 2H8 blocks acute inflammation.
면역원
D10.G4.1 murine T cells (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843 -854).
Epitope: Extracellular domain.
애플리케이션
Anti-PECAM-1 Antibody, clone 2H8, Azide Free detects level of PECAM-1 & has been published & validated for use in Elecron Microscopy, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Neutralization Assay.
Immunofluorescence Analysis: Representative lots immunostained endothelial blood vessel cells in 4% paraformaldehyde-fixed frozen tumor sections from mice and dogs as a means for assessing lymph vessel density in tumor xenografts by fluorescent immunohistochemistry (Donat, U., et al. (2012). PLoS One. 7(9):e45942; Gentschev, I., et al. (2012). PLoS One.7(5):e37239).
Immunofluorescence Analysis: A representative lot immunostained endothelial cells in formalin-fixed, paraffin-embedded murine peritoneum sections by fluorescent immunohistochemistry (Tanabe, K., et al. (2007). Kidney Int. 71(3):227-238).
Immunocytochemistry Analysis: A representative lot immunostained 4% paraformaldehyde-fixed endothelial cells differentiated from murine ESCs in cultures (Purpura, K.A., et al. (2008). Stem Cells. 26(11):2832-2842).
Immunocytochemistry Analysis: Clone 2H8 immunostained 3% paraformaldehyde-fixed, 0.5% NP-40-permeabilized COS-7 transfectants expressing murine, but not human, CD31 by fluorescent immunocytochemistry (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
Neutralization Analysis: A representative lot, when injected intravenously in mice, blocked leukocyte emigration in response to thioglycollate injection (Bogen, S., et al. (1994). J. Exp. Med. 179(3):1059-1064).
Neutralization Analysis: A representative lot blocked murine PECAM-mediated aggregation of mouse L cells (Xie, Y., and Muller, W.A. (1993). Proc. Natl. Acad. Sci. U. S. A. 190(12):5569-5573).
Flow Cytometry Analysis: A representative lot detected the expression of exogenously transfected murine CD31 on the surface of mouse L cells (Xie, Y., and Muller, W.A. (1993). Proc. Natl. Acad. Sci. U. S. A. 190(12):5569-5573).
Flow Cytometry Analysis: Clone 2H8 detected CD31 immunoreactivity on the surface of various murine B- and T-cell lines (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
Immunoprecipitation Analysis: A representative lot immunoprecipitated exogenously expressed murine, but not human, CD31 from COS-7 transfectants (Xie, Y., and Muller, W.A. (1993). Proc. Natl. Acad. Sci. U. S. A. 190(12):5569-5573).
Immunoprecipitation Analysis: Clone 2H8 immunoprecipitated CD31 from murine CTLL T lymphocytes and Sp2/0 B lymphocytes (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
Electron Microscopy Analysis: Clone 2H8 was biotinylated and immunostained lymphocytes in 0.01% glutaraldehyde-/2% formaldehyde-fxed frozen reactive lymph node tissues from KLH-immunized mice (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
Immunohistochemistry Analysis: Clone 2H8 immunostained lymphocytes adherent to the sinusoidal and postcapillary venular endothelium of frozen reactive lymph node tissues from KLH-immunized mice (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
Immunofluorescence Analysis: A representative lot immunostained endothelial cells in formalin-fixed, paraffin-embedded murine peritoneum sections by fluorescent immunohistochemistry (Tanabe, K., et al. (2007). Kidney Int. 71(3):227-238).
Immunocytochemistry Analysis: A representative lot immunostained 4% paraformaldehyde-fixed endothelial cells differentiated from murine ESCs in cultures (Purpura, K.A., et al. (2008). Stem Cells. 26(11):2832-2842).
Immunocytochemistry Analysis: Clone 2H8 immunostained 3% paraformaldehyde-fixed, 0.5% NP-40-permeabilized COS-7 transfectants expressing murine, but not human, CD31 by fluorescent immunocytochemistry (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
Neutralization Analysis: A representative lot, when injected intravenously in mice, blocked leukocyte emigration in response to thioglycollate injection (Bogen, S., et al. (1994). J. Exp. Med. 179(3):1059-1064).
Neutralization Analysis: A representative lot blocked murine PECAM-mediated aggregation of mouse L cells (Xie, Y., and Muller, W.A. (1993). Proc. Natl. Acad. Sci. U. S. A. 190(12):5569-5573).
Flow Cytometry Analysis: A representative lot detected the expression of exogenously transfected murine CD31 on the surface of mouse L cells (Xie, Y., and Muller, W.A. (1993). Proc. Natl. Acad. Sci. U. S. A. 190(12):5569-5573).
Flow Cytometry Analysis: Clone 2H8 detected CD31 immunoreactivity on the surface of various murine B- and T-cell lines (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
Immunoprecipitation Analysis: A representative lot immunoprecipitated exogenously expressed murine, but not human, CD31 from COS-7 transfectants (Xie, Y., and Muller, W.A. (1993). Proc. Natl. Acad. Sci. U. S. A. 190(12):5569-5573).
Immunoprecipitation Analysis: Clone 2H8 immunoprecipitated CD31 from murine CTLL T lymphocytes and Sp2/0 B lymphocytes (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
Electron Microscopy Analysis: Clone 2H8 was biotinylated and immunostained lymphocytes in 0.01% glutaraldehyde-/2% formaldehyde-fxed frozen reactive lymph node tissues from KLH-immunized mice (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
Immunohistochemistry Analysis: Clone 2H8 immunostained lymphocytes adherent to the sinusoidal and postcapillary venular endothelium of frozen reactive lymph node tissues from KLH-immunized mice (Bogen, S.A., et al. (1992). Am. J. Pathol. 141(4):843-854).
Research Category
Cell Structure
Cell Structure
Research Sub Category
Adhesion (CAMs)
Adhesion (CAMs)
결합
Replaces: 04-1074
물리적 형태
Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, containing no preservatives.
Protein A purified
저장 및 안정성
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
분석 메모
Control
Mouse cell lung tissue
Mouse cell lung tissue
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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문서
Flow cytometry dye selection tips match fluorophores to flow cytometer configurations, enhancing panel performance.
Troubleshooting guide offers solutions for common flow cytometry problems, ensuring improved analysis performance.
프로토콜
Learn key steps in flow cytometry protocols to make your next flow cytometry experiment run with ease.
Explore our flow cytometry guide to uncover flow cytometry basics, traditional flow cytometer components, key flow cytometry protocol steps, and proper controls.
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