MAB360

Anti-Glial Fibrillary Acidic Protein Antibody

CHEMICON^®, mouse monoclonal, GA5

• 동의어(들): GFAP
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 제품명: Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5, ascites fluid, clone GA5, Chemicon^®
• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: ascites fluid
• 항체 생산 유형: primary antibodies
• 클론: GA5, monoclonal
• 종 반응성: rat, human, mouse, rabbit, chicken, pig, bovine
• 포장: antibody small pack of 25 μL
• 제조업체/상표: Chemicon^®
• 기술: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• 동형: IgG1
• NCBI 수납 번호: NM_002055.2
• UniProt 수납 번호: P14136
• 배송 상태: ambient
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... GFAP(2670)

설명

일반 설명

Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.

특이성

Glial fibrillar acidic protein. On western blots of extracts from a human glioma cell line (U33CG/343MG), MAB360 recognizes a band at approximately 51 kDa corresponding to GFAP (Debus, 1983). By immunohistochemistry it recognizes astrocytes and Bergmann glia cells, glioma and glial cell derived tumors. Shows no cross-reactivity with vimentin.

Reactivity with other species has not been determined.

면역원

Purified GFAP from porcine spinal cord.

애플리케이션

Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 detects level of Glial Fibrillary Acidic Protein & has been published & validated for use in IC, IH, IH(P) & WB with more than 65 product citations.

Immunocytochemistry:
A previous lot was used on cultured mammalian cells.

Immunohistochemistry(paraffin):
1:400-1:800 dilution from a previous lot was used on alcohol-fixed paraffin embedded sections of rat brain (cerebrum or cerebellum) and human brain.

Immunoblotting: A 1:1000 dilution of a previous lot was used.

Optimal working dilutions must be determined by the end user

품질

Routinely evaluated by Western Blot on Mouse brain lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected GFAP on 10 μg of Mouse brain lysates.

표적 설명

~ 51 kDa

결합

Replaces: 04-1031; 04-1062

물리적 형태

Unpurified mouse monoclonal IgG1 liquid in buffer containing 15 mM sodium azide.

분석 메모

Control
Positive Control: Cultured astrocytes, brain tissue (white matter), spinal chord, retina

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable