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Merck
모든 사진(4)

주요 문서

MABN1101

Sigma-Aldrich

Anti-SRY, clone 1G4 Antibody

clone 1G4, from mouse

동의어(들):

Sex-determining region Y protein, Testis-determining factor, SRY

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

1G4, monoclonal

종 반응성

human

기술

ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable

동형

IgG1

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... SRY(6736)

관련 카테고리

일반 설명

Sex-determining region Y protein, also known as Testis-determining factor, and encoded by the human gene SRY or TDF is a transcriptional regulator that controls a genetic switch in male development. SRY is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) to Sertoli rather than granulosa cells. In male adult brain SRY is involved in the maintenance of motor functions of dopaminergic neurons. SRY is also involved in different aspects of gene regulation including promoter activation or repression, DNA bending and manipulation. The SRY HMG box recognizes DNA by partial intercalation into the minor groove, and SRY is also involved in pre-mRNA splicing. SRY is expressed in the developing testis and surrounding tissue and can be found expressed in neurons in the adult. SRY is nuclear associated.

면역원

Purified recombinant fragment of human SRY expressed in E. Coli

애플리케이션

Anti-SRY, clone 1G4 Antibody is an antibody against SRY for use in western blotting, IHC, flow cytometry & ELISA.
Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected SRY in cervical and ovarian cancer tissues.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected SRY in HepG2 cells.

ELISA: A representative lot detected control Antigen (100 ng) and Antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

품질

Evaluated by Western Blotting in NTERA-2 cell lysate.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected SRY in NTERA-2 cell lysate.

표적 설명

~36 kDa observed. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified
Protein G Purified
Purified mouse monoclonal in PBS containing up to 0.1% sodium azide and 0.5% protein stabilizer.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

Eugene Lee et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 37(7), 1382-1399 (2022-04-25)
Poor survival of grafted cells is the major impediment of successful cell-based therapies for bone regeneration. Implanted cells undergo rapid death in an ischemic environment largely because of hypoxia and metabolic stress from glucose deficiency. Understanding the intracellular metabolic processes

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