추천 제품
생물학적 소스
rat
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
mAB11, monoclonal
종 반응성
human, mouse
기술
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
동형
IgG2aκ
NCBI 수납 번호
UniProt 수납 번호
배송 상태
ambient
타겟 번역 후 변형
unmodified
유전자 정보
human ... ITGA5(3678)
mouse ... Itga5(16402)
일반 설명
Integrin alpha-5 (UniProt: P08648; also known as CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD49e) is encoded by the ITGA5 (also known as FNRA) gene (Gene ID: 3678) in human. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. Integrin alpha-5 is a single-pass type I membrane protein is expressed on thymocytes, T cells, monocytes, platelets, early B cells, and activated in B cells. It is composed of a light and heavy chain. It contains four calcium binding sites (aa 480-488; 334-342; 401-409; and 465-473). Structurally, it consists of an extracellular domain (aa 4-995), a transmembrane domain (aa 996-1021), and a cytoplasmic domain (aa 1022-1049). When associated with beta 1 subunit it can serves as a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. Integrin alpha-5 is also expressed in articular cartilage and plays a role in synovial joint development and in the regulation of chondrocyte survival and matrix degradation in articular cartilage. Membrane fractions obtained from fibroblasts of diabetic animals display significant reduction in integrin alpha-5 levels. (Ref.: Almeida, ME et al., (2016). Mol. Cell. Biochem. 421(1-2); 19-28).
특이성
Clone mAB11 is a rat monoclonal antibody that specifically detects Integrin alpha 5 in human and murine cells.
면역원
Fibronectin receptor purified from human placenta.
애플리케이션
Anti-Integrin alpha5 (CD49e), clone mAb11, Cat. No. MABT822 is a rat monoclonal antibody that detects Integrin alpha 5 and has been tested for use in Immunocytochemistry and Immunoprecipitation.
Immunocytochemistry Analysis: A representative lot detected Integrin alpha5 (CD49e) in Immunocytochemistry applications (LaFlamme, S.E., et. al. (1992). J Cell Biol. 117(2):437-47; Clark, K., et. al. (2005). J Cell Sci. 118(Pt 2):291-300; Joo, E.E., et. al. (2014). Nat Commun. 5:3510).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Integrin alpha5 (CD49e) in Immunoprecipitation applications (LaFlamme, S.E., et. al. (1992). J Cell Biol. 117(2):437-47).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Integrin alpha5 (CD49e) in Immunoprecipitation applications (LaFlamme, S.E., et. al. (1992). J Cell Biol. 117(2):437-47).
Research Category
Cell Structure
Cell Structure
품질
Evaluated by Immunocytochemistry in human Foreskin Fibroblast (HFF) cells.
Immunocytochemistry Analysis): A 1:25 dilution of this antibody detected Integrin alpha5 (CD49e) in human Foreskin Fibroblast (HFF) cells.
Immunocytochemistry Analysis): A 1:25 dilution of this antibody detected Integrin alpha5 (CD49e) in human Foreskin Fibroblast (HFF) cells.
표적 설명
114.54 kDa calculated.
물리적 형태
Format: Purified
Protein G purified
Purified rat monoclonal antibody IgG2a in PBS without azide.
저장 및 안정성
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
기타 정보
Concentration: Please refer to lot specific datasheet.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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Christoph J Burckhardt et al.
Developmental cell, 56(8), 1164-1181 (2021-03-25)
Cells probe their surrounding matrix for attachment sites via integrins that are internalized by endocytosis. We find that SH3BP4 regulates integrin surface expression in a signaling-dependent manner via clathrin-coated pits (CCPs). Dephosphorylated SH3BP4 at S246 is efficiently recruited to CCPs
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