추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
Mobu-1, monoclonal
양식
liquid
포함
≤0.1% sodium azide as preservative
종 반응성(상동성에 의해 예측)
all
제조업체/상표
Calbiochem®
저장 조건
do not freeze
동형
IgG1
배송 상태
wet ice
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
일반 설명
Anti-BrdU (Ab-3), mouse monoclonal, clone Mobu-1, recognizes BrdU. Requires biosynthetic labeling of the target cells with BrdU. It is validated for ICC and use with frozen and paraffin sections.
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with P3-X63-Ag8-653 mouse myeloma cells (see application references). Recognizes BrdU (bromodeoxyuridine).
Recognizes BrdU (bromodeoxyuridine). Use of this antibody first requires biosynthetic labeling of the target cells with bromodeoxyuridine. This is the preferred BrdU antibody for staining.
면역원
BrdU-labeled DNA
애플리케이션
Frozen Sections (2 g/ml)
Immunocytochemistry (1 g/ml)
Paraffin Sections (2.5 g/ml, trysin pre-treatment required)
Immunoblotting (not recommended)
포장
Please refer to vial label for lot-specific concentration.
경고
Toxicity: Standard Handling (A)
물리적 형태
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
분석 메모
Negative Control
Unlabeled cells
Unlabeled cells
Positive Control
BrdU labeled DNA
BrdU labeled DNA
기타 정보
Gratzner, H.G. 1982. Science218, 474.
Gratzner, H.G. and Leif, R.C. 1981. Cytometry1, 385.
Gratzner, H.G. et al. 1975. Exp. Cell Res.95, 88.
Gratzner, H.G. and Leif, R.C. 1981. Cytometry1, 385.
Gratzner, H.G. et al. 1975. Exp. Cell Res.95, 88.
To enable antibody binding to the incorporated BrdU, cells must be fixed, permeabilized, and the DNA denatured. This is done in one step by treatment with Fixative/Denaturing Solution (70% EtOH, 0.1 N NaOH). Use of this antibody first requires biosynthetic labeling of the target cells with bromodeoxyuridine (Cat. No. 203806). Cultured cells can be pulse labeled with 10 µM bromodeoxyuridine for 30 minutes. For whole tissues, animals can be injected with 50 mg BrdU/kg body weight, sacrificed 1 h later, and the target organ(s) removed. Antibody should be titrated for optimal results in individual system.
법적 정보
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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